The HLA-B*2705 peptidome

Arthritis and Rheumatism
Lilach Ben DrorArie Admon

Abstract

The HLA-B27 allele is strongly associated with the group of inflammatory diseases known as the spondylarthritides (SpA). The aim of this study was to perform a large-scale, direct biochemical analysis of the HLA-B*2705 peptidome in order to identify candidates for mimicry between HLA-B27 peptides derived from cartilage proteins and arthritogenic bacterial sequences and to refine the consensus binding motif of this important allele. The peptides were recovered by recombinant expression of soluble HLA-B27 molecules secreted from cultured chondrocytic cells or HeLa cells. Analysis was based on capillary chromatography and tandem mass spectrometry in combination with stable isotope labeling with amino acids in cell culture or chemical labeling with iTRAQ to enhance the validity of the data. Over 1,268 B27 peptides were identified, with 569 of them at high certainty, thus enabling better refinement of the B27 motif. This enabled the prediction of both short peptides and long peptides whose middle residues likely bulge out of the binding groove. Moreover, we identified a number of human B27 peptide sequences derived from human cartilage proteins, some of which are similar to common bacterial sequences. The peptides we identified may ...Continue Reading

Citations

Oct 5, 2010·The Journal of Biological Chemistry·Begoña Galocha, José A López de Castro
Jul 19, 2012·The Journal of Biological Chemistry·Adriana MagnaccaRosa Sorrentino
Oct 5, 2011·Molecular & Cellular Proteomics : MCP·Noel García-MedelJosé A López de Castro
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Nov 15, 2018·Frontiers in Immunology·José A López de Castro
Oct 24, 2020·Biomolecules·Aigul Sharip, Jeannette Kunz
Nov 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandr KuznetsovGeorgij Arapidi
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