The HLA-DRalpha chain is modified by polyubiquitination.

The Journal of Biological Chemistry
Nicolas LapaqueAdrian P Kelly

Abstract

Ubiquitination plays a major role in regulating cell surface and intracellular localization of major histocompatibility complex class II molecules. Two E3 ligases, MARCH I and MARCH VIII, have been shown to polyubiquitinate lysine residue 225 in the cytoplasmic tail of I-Abeta and HLA-DRbeta. We show that lysine residue 219 in the cytoplasmic tail of DRalpha is also subject to polyubiquitination. Each chain of the HLA-DR heterodimer is independently recognized and ubiquitinated, but DRbeta is more extensively modified. In the cytoplasmic tail of DRbeta lysine, residue 225 is the only residue that is absolutely required for ubiquitination; all other residues can be deleted or substituted without loss of function. In contrast, although lysine 219 is absolutely required for modification of DRalpha, other features of the DRalpha tail act to limit the extent of ubiquitination.

References

Feb 1, 1992·Current Opinion in Immunology·P Cresswell
Oct 6, 1997·The EMBO Journal·J M Galan, R Haguenauer-Tsapis
Dec 19, 1998·The EMBO Journal·A Ciechanover
Jun 8, 1999·European Journal of Immunology·J Neefjes
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M van LithJ Neefjes
Jan 16, 2002·Current Opinion in Immunology·Elizabeth M Hiltbold, Paul A Roche
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·David J KatzmannScott D Emr
Jul 23, 2003·Nature Biotechnology·Junmin PengSteven P Gygi
Nov 24, 2004·Current Opinion in Chemical Biology·Cecile M Pickart, David Fushman
Feb 17, 2005·Journal of Biochemistry·Masayuki Komada, Naomi Kitamura
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mari Ohmura-HoshinoSatoshi Ishido
Jan 27, 2007·The EMBO Journal·Yohei MatsukiSatoshi Ishido
Feb 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aude De GassartEvelina Gatti

❮ Previous
Next ❯

Citations

Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nicolas LapaqueAdrian P Kelly
Sep 8, 2011·The Journal of Biological Chemistry·Kathleen CorcoranLonnie Lybarger
Jan 1, 2013·Results in Immunology·Marie-Claude Bourgeois-DaigneaultJacques Thibodeau
Dec 4, 2012·Molecular Immunology·Malgorzata A Garstka, Jacques Neefjes
Sep 17, 2014·The Journal of Allergy and Clinical Immunology·Jean-Louis GuéantMiguel Blanca
Dec 19, 2016·New Biotechnology·Johannes BauerJ Preben Morth
Feb 13, 2013·European Journal of Immunology·Martin JahnkeAdrian P Kelly
Jan 12, 2019·The Journal of Biological Chemistry·Yanzhao ZhangKenzo Tokunaga
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sebastian TemmeNorbert Koch
Nov 7, 2012·Traffic·Adriana R MantegazzaMichael S Marks
Jun 19, 2015·Immunological Reviews·Jaehak Oh, Jeoung-Sook Shin
Jul 2, 2009·Traffic·Marie-Elaine GauvreauJacques Thibodeau
Aug 14, 2019·Frontiers in Immunology·Heng LinHong-Bing Shu
Jan 1, 2014·International Scholarly Research Notices·Tasleem SamjiRobert E Means
Mar 4, 2021·MBio·Supawadee UmthongSpyridon Stavrou
May 17, 2021·The FEBS Journal·Takuya TadaKenzo Tokunaga
Nov 30, 2010·Current Opinion in Immunology·Tineke van den HoornJacques Neefjes

❮ Previous
Next ❯

Methods Mentioned

BETA
CLIP
ubiquitination
PCR
FACS
transfections
immunoprecipitation
confocal microscopy

Software Mentioned

MIRwt
Adobe Photoshop

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.