The homeobox transcription factor Barx2 regulates plasticity of young primary myofibers.

PloS One
Robyn MeechHelen P Makarenkova

Abstract

Adult mammalian muscle retains incredible plasticity. Muscle growth and repair involves the activation of undifferentiated myogenic precursors called satellite cells. In some circumstances, it has been proposed that existing myofibers may also cleave and produce a pool of proliferative cells that can re-differentiate into new fibers. Such myofiber dedifferentiation has been observed in the salamander blastema where it may occur in parallel with satellite cell activation. Moreover, ectopic expression of the homeodomain transcription factor Msx1 in differentiated C2C12 myotubes has been shown to induce their dedifferentiation. While it remains unclear whether dedifferentiation and redifferentiaton occurs endogenously in mammalian muscle, there is considerable interest in induced dedifferentiation as a possible regenerative tool. We previously showed that the homeobox protein Barx2 promotes myoblast differentiation. Here we report that ectopic expression of Barx2 in young immature myotubes derived from cell lines and primary mouse myoblasts, caused cleavage of the syncytium and downregulation of differentiation markers. Microinjection of Barx2 cDNA into immature myotubes derived from primary cells led to cleavage and formation of ...Continue Reading

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Citations

May 21, 2013·Gene Expression Patterns : GEP·Emma JuuriFrederic Michon
Jan 12, 2012·PloS One·Nikolaos P MastroyiannopoulosLeonidas A Phylactou
Feb 20, 2013·Gene Expression Patterns : GEP·Nicholas J HudsonBrian P Dalrymple
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Nov 6, 2013·Bioscience Reports·Nikolaos P MastroyiannopoulosLeonidas A Phylactou
Mar 13, 2015·Stem Cells International·Gustavo Torres de SouzaCarlos Magno da Costa Maranduba
Dec 22, 2017·Plastic and Reconstructive Surgery. Global Open·Scott J RappBrian S Pan
Jan 11, 2013·Physiological Reviews·Hang YinMichael A Rudnicki
Oct 19, 2020·Biochemical and Biophysical Research Communications·Zijun QiaoQilong Chen
Apr 21, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Phablo Abreu

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Methods Mentioned

BETA
transfection
SMA
Co-immunoprecipitation
PCR
transfections
confocal microscopy

Software Mentioned

Exel

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