The Hos2 Histone Deacetylase Controls Ustilago maydis Virulence through Direct Regulation of Mating-Type Genes

PLoS Pathogens
Alberto Elías-VillalobosJosé I Ibeas

Abstract

Morphological changes are critical for host colonisation in plant pathogenic fungi. These changes occur at specific stages of their pathogenic cycle in response to environmental signals and are mediated by transcription factors, which act as master regulators. Histone deacetylases (HDACs) play crucial roles in regulating gene expression, for example by locally modulating the accessibility of chromatin to transcriptional regulators. It has been reported that HDACs play important roles in the virulence of plant fungi. However, the specific environment-sensing pathways that control fungal virulence via HDACs remain poorly characterised. Here we address this question using the maize pathogen Ustilago maydis. We find that the HDAC Hos2 is required for the dimorphic switch and pathogenic development in U. maydis. The deletion of hos2 abolishes the cAMP-dependent expression of mating type genes. Moreover, ChIP experiments detect Hos2 binding to the gene bodies of mating-type genes, which increases in proportion to their expression level following cAMP addition. These observations suggest that Hos2 acts as a downstream component of the cAMP-PKA pathway to control the expression of mating-type genes. Interestingly, we found that Clr3, a...Continue Reading

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Citations

Mar 17, 2018·PLoS Genetics·Celine TassetSureshkumar Balasubramanian
Mar 28, 2018·Scientific Reports·Fabiana BrandãoJ Andrew Alspaugh
Apr 16, 2019·Molecular Plant-microbe Interactions : MPMI·Huahui LanShihua Wang
May 6, 2020·Pest Management Science·Christian WinterMaria Angelica Quintero Palomar
Nov 24, 2019·The Journal of Microbiology·Jongjune LeeJunhyun Jeon
Dec 24, 2019·Frontiers in Microbiology·Ingo BauerHubertus Haas
Jan 10, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·José Ruiz-HerreraJohn Velez-Haro
Jul 28, 2020·Microorganisms·Domingo Martínez-SotoClaudia Geraldine León-Ramírez
Apr 29, 2021·Annual Review of Phytopathology·Wende LiuXiao-Lin Chen

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Methods Mentioned

BETA
ChIP
acetylation
Light microscopy
immunoprecipitation
PCR
protein assay
Optical

Software Mentioned

BLAST
MEGA5
GraphPad Prism
Adobe Photoshop
ImageJ
InterProScan Sequence Search
Metamorph

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