The Host Interactome of Spike Expands the Tropism of SARS-CoV-2.

BioRxiv : the Preprint Server for Biology
Casimir BambergerJohn R Yates

Abstract

The SARS-CoV-2 virus causes severe acute respiratory syndrome (COVID-19) and has rapidly created a global pandemic. Patients that survive may face a slow recovery with long lasting side effects that can afflict different organs. SARS-CoV-2 primarily infects epithelial airway cells that express the host entry receptor Angiotensin Converting Enzyme 2 (ACE2) which binds to spike protein trimers on the surface of SARS-CoV-2 virions. However, SARS-CoV-2 can spread to other tissues even though they are negative for ACE2. To gain insight into the molecular constituents that might influence SARS-CoV-2 tropism, we determined which additional host factors engage with the viral spike protein in disease-relevant human bronchial epithelial cells (16HBEo - ). We found that spike recruited the extracellular proteins laminin and thrombospondin and was retained in the endoplasmatic reticulum (ER) by the proteins DJB11 and FBX2 which support re-folding or degradation of nascent proteins in the ER. Because emerging mutations of the spike protein potentially impact the virus tropism, we compared the interactome of D614 spike with that of the rapidly spreading G614 mutated spike. More D614 than G614 spike associated with the proteins UGGT1, calnexi...Continue Reading

Citations

May 18, 2021·Acta Pharmaceutica Sinica. B·Zinuo ChenQinghua Cui

Methods Mentioned

BETA
immunoprecipitations
protein folding
ubiquitination
transfection
immunoprecipitation

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Donald J BentonSteven J Gamblin
MedRxiv : the Preprint Server for Health Sciences
C. Klumpp-ThomasKaitlyn Sadtler
MedRxiv : the Preprint Server for Health Sciences
C. Klumpp-ThomasKaitlyn Sadtler
© 2021 Meta ULC. All rights reserved