The House Dust Mite Major Allergen Der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity

International Archives of Allergy and Immunology
Wai Tuck SohMite Allergy Research Cohort (MARC) Study Team

Abstract

The in-depth characterization of the recently identified house dust mite (HDM) major allergen Der p 23 requires the production of its recombinant counterpart because the natural allergen is poorly extractable from fecal pellets. This study aimed to provide a detailed physico-chemical characterization of recombinant Der p 23 (rDer p 23) as well as to investigate its IgE reactivity in a cohort of HDM-allergic patients from Thailand. Purified rDer p 23, secreted from recombinant Pichia pastoris, was characterized by mass spectrometry and circular dichroism analyses as well as for its chitin-binding activity. The IgE-binding frequency and allergenicity of Der p 23 were determined by ELISA and RBL-SX38 degranulation assays, respectively. Purified intact rDer p 23 carried O-mannosylation and mainly adopted a random coil structure. Polyclonal antibodies to rDer p 23 can detect the corresponding natural allergen (nDer p 23) in aqueous fecal pellet extracts, suggesting that both forms of Der p 23 share common B-cell epitopes. Despite its homologies with chitin-binding proteins, both natural Der p 23 and rDer p 23 were unable to interact in vitro with chitin matrices. Of 222 Thai HDM-allergic patients tested, 54% displayed Der p 23-speci...Continue Reading

Citations

May 1, 2017·International Archives of Allergy and Immunology·Eduardo Santos da SilvaFatima Ferreira
May 4, 2017·International Archives of Allergy and Immunology·Angelika Tscheppe, Heimo Breiteneder
Mar 29, 2016·Allergy·P SatitsuksanoaUNKNOWN Mite Allergy Research Cohort (MARC) study team
Apr 30, 2017·The World Allergy Organization Journal·Mario Sánchez-BorgesArnaldo Capriles-Hulett
Jan 23, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T SoongrungA Jacquet
Sep 20, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Pinya PulsawatAlain Jacquet
Feb 9, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Mihaela ZidarnPeter Korošec
May 18, 2016·Current Allergy and Asthma Reports·Anna PomésSabina Wünschmann
Aug 19, 2016·Current Allergy and Asthma Reports·Emmanuel NonyPhilippe Moingeon
Sep 8, 2016·Current Allergy and Asthma Reports·Wayne R Thomas
Jan 15, 2021·Scientific Reports·Sze Lei PangFook Tim Chew
May 7, 2020·The World Allergy Organization Journal·Luis CaraballoAlexander Karaulov
Aug 23, 2021·The Journal of Allergy and Clinical Immunology·Huey-Jy HuangSusanne Vrtala

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