The housekeeping gene YWHAZ remains stable in a model of developmentally primed non-alcoholic fatty liver disease.

Liver International : Official Journal of the International Association for the Study of the Liver
Kimberley D BruceF R Cagampang

Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common form of chronic liver disease in Western society. Comparative gene expression studies are beginning to elucidate the molecular mechanisms underlying NAFLD progression. We have previously shown that high fat diets during early life can prime non-alcoholic steatohepatitis (NASH) in adulthood, through lipogenesis gene elevation. To generate accurate results in such studies, appropriate housekeeping genes (HKG) which are unaffected by disease processes, are used for data normalisation. However, there is little existing data to show the effects of NAFLD on HKG expression. To identify the HKG in a mouse model of developmentally primed NAFLD and NASH, which maintains expression stability. We determined the expression stability of six candidates HKG (GAPDH, YWHAZ, B2M, EIF4A2, ACTB and CYC1) in a mouse model of developmentally primed NAFLD in both the day and night, using geNORM qBasePlus software. HKG expression differed across dietary groups and time of day. In the majority of treatment groups and time points the most stable gene was YWHAZ. Following high fat diet interventions CYC1 became notably unstable. Overall the effect of NAFLD and NASH on HKG expression was to maint...Continue Reading

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Citations

Jan 18, 2013·PloS One·Dominic HennHans-Joachim Schäfers
Dec 3, 2014·Biochemical and Biophysical Research Communications·Kristien J A LemmensGuido R M M Haenen
Aug 18, 2017·Antioxidants & Redox Signaling·Chiara MilanesePier G Mastroberardino
Jun 13, 2019·The American Journal of Clinical Nutrition·Honghuang LinJoanne M Murabito
Feb 25, 2020·Cells·Gayane Hrachia BuniatianLusine Danielyan
Mar 2, 2019·Journal of Gastroenterology and Hepatology·Nathan KukWilliam Sievert
Feb 25, 2020·Gastroenterology Research and Practice·Layanne C C AraujoCarla R O Carvalho
Oct 19, 2020·Transboundary and Emerging Diseases·Kátia R GrochJosé L Catão-Dias

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