The Hox Gene egl-5 Acts as a Terminal Selector for VD13 Development via Wnt Signaling

Journal of Developmental Biology
Meagan KurlandBrian D Ackley

Abstract

Nervous systems are comprised of diverse cell types that differ functionally and morphologically. During development, extrinsic signals, e.g., growth factors, can activate intrinsic programs, usually orchestrated by networks of transcription factors. Within that network, transcription factors that drive the specification of features specific to a limited number of cells are often referred to as terminal selectors. While we still have an incomplete view of how individual neurons within organisms become specified, reporters limited to a subset of neurons in a nervous system can facilitate the discovery of cell specification programs. We have identified a fluorescent reporter that labels VD13, the most posterior of the 19 inhibitory GABA (γ-amino butyric acid)-ergic motorneurons, and two additional neurons, LUAL and LUAR. Loss of function in multiple Wnt signaling genes resulted in an incompletely penetrant loss of the marker, selectively in VD13, but not the LUAs, even though other aspects of GABAergic specification in VD13 were normal. The posterior Hox gene, egl-5, was necessary for expression of our marker in VD13, and ectopic expression of egl-5 in more anterior GABAergic neurons induced expression of the marker. These result...Continue Reading

References

Nov 1, 1978·The Journal of Physiology·L Landmesser
Nov 1, 1978·The Journal of Physiology·L Landmesser
May 1, 1974·Genetics·S Brenner
Jul 22, 1993·Nature·S L McIntireH R Horvitz
Dec 31, 1997·Nature·S L McIntireE M Jorgensen
Aug 5, 2009·Current Topics in Developmental Biology·Jeremy S Dasen, Thomas M Jessell
Dec 24, 2010·Science·Mark B GersteinRobert H Waterston
Nov 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Irini Topalidou, Martin Chalfie
Nov 12, 1986·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G WhiteS Brenner
Jun 18, 2015·Nature·Kelly HowellOliver Hobert
Mar 13, 2016·Current Topics in Developmental Biology·Oliver Hobert
Sep 17, 2016·Developmental Biology·Richard F Campbell, Walter W Walthall
Apr 26, 2018·Current Opinion in Neurobiology·Clinton Cave, Shanthini Sockanathan
Apr 8, 2019·Current Opinion in Neurobiology·Peter J Osseward, Samuel L Pfaff

❮ Previous
Next ❯

Citations

Dec 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zohar AbergelEinav Gross

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation

Software Mentioned

ImageJ
GraphPad QuickCalcs
Prism GraphPad
Fluoview
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.