The human allergens of mesquite (Prosopis juliflora)

Clinical and Molecular Allergy : CMA
Sue Killian, John McMichael

Abstract

BACKGROUND: A computerized statistical analysis of allergy skin test results correlating patient reactivities initiated our interest in the cross-reactive allergens of mesquite tree pollen. In-vitro testing with mesquite-sensitized rabbits and a variety of deciduous tree pollens revealed so many cross-reactivities that it became apparent there could be more allergens in mesquite than previously described in the world literature. Our purpose was to examine the allergens of mesquite tree pollen (Prosopis juliflora) which elicit an IgE response in allergic humans so that future research could determine if these human allergens cross-react with various tree pollens in the same manner as did the mesquite antiserum from sensitized rabbits. METHODS: Proteins from commercial mesquite tree pollen were separated by polyacrylamide gel electrophoresis in the presence of sodium-dodecyl-sulphate. These mesquite proteins were subjected to Western blotting using pooled sera from ten mesquite-sensitive patients and goat anti-human IgE. The allergens were detected using an Amplified Opti-4-CN kit, scanned, and then interpreted by Gel-Pro software. RESULTS: Thirteen human allergens of mesquite pollen were detected in this study. CONCLUSION: The n...Continue Reading

References

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Citations

Jan 18, 2018·Neurotoxicity Research·Victor Diogenes Amaral da SilvaSilvia Lima Costa
Oct 8, 2008·European Journal of Clinical Investigation·A DhyaniS Sridhara
Jan 26, 2020·Plants·M Iftikhar HussainLuís González

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Methods Mentioned

BETA
electrophoresis
gel

Software Mentioned

Pro
Gel Pro
Gel

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