Abstract
Thymocytes interact with various subpopulations of thymic epithelial cells (TECs) at different stages of their development. To identify new molecules specifically expressed in TECs and/or thymic nurse cells (TNCs), we used representational difference analysis. We identified a LIM protein located on mouse chromosome 17 (m17TLP) and belonging to the family of the LIM-only proteins (LIMo). We found a new splice variant in addition to the two described A and B isoforms. The three alternative species of m17TLP are found strictly in the thymic stroma. This protein is expressed on a subpopulation of TECs and TNCs. Strikingly, we found that the human ortholog of m17TLP, located on chromosome 6 (h6LIMo), is expressed in most tissues, but not in skeletal muscle. We have identified four human splice variants of h6LIMo which differ in their carboxy-terminal regions. The sequence comprising the genomic structure suggests that CRP2 is the closest known relative of m17TLP. Although the human and mouse nucleotide sequences are 88-97% homologous, this homology is reduced to 47% in the promoter regions, which strongly suggests that their differential expression is related to their promoter regulatory activity.
References
Dec 1, 1992·The Journal of Cell Biology·I SadlerM C Beckerle
Aug 1, 1990·The Journal of Experimental Medicine·J PenningerG Wick
Jan 1, 1985·European Journal of Immunology·P Andrews, R L Boyd
Feb 9, 1989·Nature·R G Lorenz, P M Allen
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B W Glickman, L S Ripley
Apr 1, 1980·The Journal of Experimental Medicine·H WekerleM Ernst
Dec 25, 1994·Nucleic Acids Research·M Hubank, D G Schatz
Jan 1, 1995·Cellular Immunology·C HiramineK Hojo
Oct 21, 1994·Cell·K L Schmeichel, M C Beckerle
Sep 1, 1994·Trends in Genetics : TIG·I Sánchez-García, T H Rabbitts
Sep 1, 1993·Immunology Today·R L BoydP Hugo
Feb 12, 1993·Science·N LisitsynM Wigler
Sep 1, 1996·Mutagenesis·A M OsterholmS M Hou
Aug 22, 1997·Journal of Immunological Methods·J K FrazerJ D Capra
Oct 27, 1997·The Journal of Biological Chemistry·H A LouisM C Beckerle
Dec 12, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M C Beckerle
Aug 29, 1998·The Journal of Biological Chemistry·R KonratK Bister
Nov 14, 1998·Circulation Research·M K JainM E Lee
Mar 25, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J R HendersonM C Beckerle
May 8, 1999·Biochemistry·X YaoM C Beckerle
Mar 8, 2000·Mechanisms of Development·I Bach
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·M PezzanoJ Guyden
Nov 20, 2001·Molecular and Cellular Biology·J KirchnerM J Bevan