PMID: 8610099Apr 2, 1996Paper

The human CAS protein which is homologous to the CSE1 yeast chromosome segregation gene product is associated with microtubules and mitotic spindle

Proceedings of the National Academy of Sciences of the United States of America
U ScherfU Brinkmann

Abstract

Human CAS cDNA contains a 971-aa open reading frame that is homologous to the essential yeast gene CSE1. CSE1 is involved in chromosome segregation and is necessary for B-type cyclin degradation in mitosis. Using antibodies to CAS, it was shown that CAS levels are high in proliferating and low in nonproliferating cells. Here we describe the distribution of CAS in cells and tissues analyzed with antibodies against CAS. CAS is an approximately 100-kDa protein present in the cytoplasm of proliferating cells at levels between 2 x 10(5) and 1 x 10(6) molecules per cell. The intracellular distribution of CAS resembles that of tubulin. In interphase cells, anti-CAS antibody shows microtubule-like patterns and in mitotic cells it labels the mitotic spindle. CAS is removed from microtubules by mild detergent treatment (cytoskeleton preparations) and in vincristine- or taxol-treated cells. CAS is diffusely distributed in the cytoplasm with only traces present in tubulin paracrystals or bundles. Thus, CAS appears to be associated with but not to be an integral part of microtubules. Immunohistochemical staining of frozen tissues shows elevated amounts of CAS in proliferating cells such as testicular spermatogonia and cells in the basal lay...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·U BrinkmannI Pastan
Jan 1, 1990·Annual Review of Biochemistry·R B Vallee, H S Shpetner
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·U BrinkmannI Pastan
Nov 24, 1995·Science·Z XiaM E Greenberg
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·A V GudkovI B Roninson

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Citations

May 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M de MiguelP J Donovan
Jun 8, 2002·Biochemical and Biophysical Research Communications·Gemma PujolAurelian Radu
Jan 21, 2004·Nature Reviews. Cancer·Tweeny R KauPamela A Silver
May 15, 2008·Nature Reviews. Molecular Cell Biology·Paul R Clarke, Chuanmao Zhang
Dec 18, 1998·The Journal of Biological Chemistry·J K Hood, P A Silver
Jul 4, 2008·Journal of Experimental & Clinical Cancer Research : CR·Ching-Fong LiaoMing-Chung Jiang
Aug 13, 2010·Journal of Experimental & Clinical Cancer Research : CR·Cheng-Jeng TaiMing-Chung Jiang
Jun 20, 2012·Cellular Oncology (Dordrecht)·Anke H Sillars-HardebolRemond J A Fijneman
Jul 1, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoshi OkimotoSatoshi Tashiro
Sep 7, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ming-Chung Jiang
Oct 30, 2004·Scandinavian Journal of Gastroenterology·D E AustG B Baretton
Dec 18, 2007·Journal of Lipid Research·Randolph S FaustinoGrant N Pierce
Oct 17, 1998·Molecular and Cellular Biology·J SolsbacherG Schlenstedt
Mar 6, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Annalisa LorenzatoMaria Flavia Di Renzo
Nov 30, 2018·Cell Proliferation·Chunyan LiuChengliang Xiong
Jun 10, 1998·Biochemical and Biophysical Research Communications·A KadiJ Luis
Jul 22, 2021·Journal of Developmental Biology·Lauren E BlizzardRolf W Stottmann
Nov 15, 1996·Science·J M PetersM W Kirschner

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