The human CENP-A centromeric nucleosome-associated complex

Nature Cell Biology
Daniel R FoltzDon W Cleveland

Abstract

The basic element for chromosome inheritance, the centromere, is epigenetically determined in mammals. The prime candidate for specifying centromere identity is the array of nucleosomes assembled with CENP-A, the centromere-specific histone H3 variant. Here, we show that CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of three new human centromere proteins (CENP-M, CENP-N and CENP-T), along with CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Facilitates chromatin transcription (FACT) and nucleophosmin-1 (previously implicated in transcriptional chromatin remodelling and as a multifunctional nuclear chaperone, respectively) are absent from histone H3-containing nucleosomes, but are stably recruited to CENP-A nucleosomes independent of CENP-A NAC. Seven new CENP-A-nucleosome distal (CAD) centromere components (CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S) are identified as assembling on the CENP-A NAC. The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint sig...Continue Reading

References

Jun 1, 1991·The Journal of Cell Biology·R P ZinkowskiB R Brinkley
Apr 1, 1987·The Journal of Cell Biology·W C EarnshawD W Cleveland
Oct 24, 1998·The Journal of Cell Biology·R H ChenA W Murray
Sep 17, 1999·The Journal of Biological Chemistry·N SugataK Todokoro
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·E V HowmanK H Choo
Aug 3, 2001·Nature Reviews. Genetics·B A SullivanG H Karpen
Jan 5, 2002·The Journal of Cell Biology·I M CheesemanG Barnes
Mar 7, 2002·Developmental Cell·Michael D BlowerGary H Karpen
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Apr 17, 2002·The Journal of Cell Biology·Iain M CheesemanGeorjana Barnes
Apr 24, 2002·Developmental Cell·Ai NishihashiTatsuo Fukagawa
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J MacCossJohn R Yates
Aug 28, 2002·American Journal of Human Genetics·David J Amor, K H Andy Choo
Sep 6, 2002·Oncogene·Masaru Okuda
Aug 23, 2003·Science·Rimma BelotserkovskayaDanny Reinberg
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Andrew D McAinshPeter K Sorger
Feb 12, 2004·Methods in Enzymology·Kinya YodaKeiji Hashimoto
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Christèle Maison, Geneviève Almouzni
Jul 13, 2004·Trends in Cell Biology·David J AmorK H Andy Choo
Jul 30, 2004·Nature·Ben E BlackDon W Cleveland
Jan 13, 2005·Science's STKE : Signal Transduction Knowledge Environment·Iain M Cheeseman, Arshad Desai
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Kavitha Sarma, Danny Reinberg
May 5, 2005·Molecular and Cellular Biology·Vinciane RégnierWilliam Brown
Sep 8, 2005·The Journal of Cell Biology·Yinghui MaoDon W Cleveland
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Steven Henikoff, Kami Ahmad
Nov 17, 2005·Molecular and Cellular Biology·Yukinori MinoshimaTatsuo Fukagawa

❮ Previous
Next ❯

Citations

Jun 18, 2009·European Biophysics Journal : EBJ·D HellwigStephan Diekmann
Sep 24, 2010·Chromosoma·Bernardo Orr, Claudio E Sunkel
Jul 14, 2011·Chromosoma·Marinela Perpelescu, Tatsuo Fukagawa
May 4, 2012·Chromosoma·Steven Henikoff, Takehito Furuyama
Jun 30, 2010·Molecular Genetics and Genomics : MGG·Gunjan D MehtaSantanu Kumar Ghosh
Jun 27, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tetsuya Hori, Tatsuo Fukagawa
Jun 29, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Luis P ValenteLars E T Jansen
Jul 25, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jan H BergmannWilliam C Earnshaw
Jul 18, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Kaitlin M StimpsonBeth A Sullivan
Apr 13, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·W C EarnshawD W Cleveland
Nov 6, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Kristin C Scott
Apr 9, 2013·Progress in Biophysics and Molecular Biology·Sergej TschernyschkowBashar Ibrahim
Mar 14, 2013·Trends in Cell Biology·Stefan Westermann, Alexander Schleiffer
Apr 20, 2013·Trends in Biochemical Sciences·Luying JiaHongtao Yu
Aug 6, 2013·Trends in Genetics : TIG·William C Earnshaw, Don W Cleveland
Jun 25, 2008·Cell Research·Daniel Figeys
May 13, 2006·Nature Cell Biology·Barbara MelloneGary H Karpen
Jul 2, 2009·Nature Cell Biology·Nikolina Sekulic, Ben E Black
Jun 23, 2009·Nature Cell Biology·Christopher W CarrollAaron F Straight
Mar 17, 2010·Nature Cell Biology·Ana C AmaroPatrick Meraldi
May 9, 2012·Nature Cell Biology·Alexander SchleifferStefan Westermann
Jul 23, 2013·Nature Cell Biology·Daniele FachinettiDon W Cleveland
May 19, 2011·Nature Communications·Mekonnen Lemma DechassaKarolin Luger
Nov 13, 2008·Nature Reviews. Genetics·Robin C Allshire, Gary H Karpen
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Apr 22, 2011·Nature Reviews. Molecular Cell Biology·Jolien S Verdaasdonk, Kerry Bloom
Jul 15, 2009·Nature Structural & Molecular Biology·Sang-Moon YunKyung S Lee
Jun 7, 2013·Nature Structural & Molecular Biology·Elaine M DunleavyGary H Karpen
May 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tamás FischerShiv I S Grewal
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Samantha G ZeitlinDon W Cleveland
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Muhammad ShuaibAli Hamiche
May 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Susana Abreu RibeiroWilliam C Earnshaw
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Emilios K DimitriadisYamini Dalal
Apr 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jung-Eun ParkKyung S Lee
May 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Damien Goutte-GattatStefan Dimitrov
Jun 26, 2009·The Journal of Biological Chemistry·Kai YuanXuebiao Yao
Nov 17, 2009·The Journal of Biological Chemistry·Haitong HouSongtao Jia
Nov 27, 2009·The Journal of Biological Chemistry·Joseph A GoldmanRobert E Kingston
Feb 20, 2010·The Journal of Biological Chemistry·Akihisa OsakabeHitoshi Kurumizaka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.