The human chromosomal gene for necdin, a neuronal growth suppressor, in the Prader-Willi syndrome deletion region

Gene
Y NakadaK Yoshikawa

Abstract

Necdin is a growth suppressor expressed in virtually all postmitotic neurons in the brain. The human necdin gene, NDN, is maternally imprinted and deleted in the Prader-Willi syndrome, a neurobehavioral contiguous gene disorder. Here, we isolated and characterized the human chromosomal necdin gene and its promoter region. The necdin gene is intronless, and it encodes a protein of 321 amino acid residues, four residues shorter than mouse Necdin. By fluorescence in-situ hybridization analysis, the necdin gene was localized to chromosome 15q11.2-q12 within the Prader-Willi syndrome deletion region. CpG islands were found in a region extending from the proximal 5'-flanking sequence to the protein coding region. The 5'-flanking sequence, which lacks canonical TATA and CAAT boxes, possessed a promoter activity in postmitotic neurons derived from murine embryonal carcinoma P19 cells. Methylation in vitro of HhaI CpG sites in the promoter region reduced the transcriptional activity. These results suggest that the necdin gene is silenced through methylation of the CpG island encompassing its promoter region.

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Citations

May 10, 2000·Neuroscience Research·K Yoshikawa
May 20, 2000·The Journal of Perinatal & Neonatal Nursing·C A Prows, R J Hopkin
Jun 10, 2014·PloS One·Ibrahim GurKazuaki Yoshikawa
Jan 23, 1999·Current Opinion in Genetics & Development·L D Hurst, G T McVean
Dec 17, 2009·Biological Psychology·Jorge Gallego, Boris Matrot
Jan 26, 2005·Journal of Cellular Biochemistry·Meredith L HanelRachel Wevrick
May 15, 2008·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·R OgawaY Fujii
Nov 7, 2006·Gene Expression Patterns : GEP·Isao NishimuraKazuaki Yoshikawa
Dec 24, 2013·Clinical Lymphoma, Myeloma & Leukemia·Kayo Harada-ShiradoYasuchika Takeishi
Dec 4, 2004·Journal of Cellular Biochemistry·Hideo TaniuraKazuaki Yoshikawa
Mar 21, 2002·Cancer Investigation·Alexei F KirkinJesper Zeuthen
Oct 16, 2014·Journal of Child Neurology·Sen LiuZhihong Wu
Oct 3, 1999·Nature Genetics·M GérardC L Stewart
Jul 6, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·F OeffnerK H Grzeschik
Sep 22, 2006·Human Molecular Genetics·N Carolyn Schanen
Jan 26, 2008·Journal of Applied Physiology·C Gaultier, J Gallego
Aug 29, 2002·The Journal of Biological Chemistry·Masakatsu KobayashiKazuaki Yoshikawa
May 29, 1999·The Journal of Biological Chemistry·H TaniuraK Yoshikawa
Nov 1, 2003·The Journal of Biological Chemistry·Ken-ichiro KuwakoKazuaki Yoshikawa
Mar 23, 2017·American Journal of Medical Genetics. Part a·Ruqaiah AltassanSalma M Wakil
Jun 29, 2004·World Journal of Gastroenterology : WJG·Jiang Xiao, Hong-Song Chen
Jun 27, 2002·Experimental Cell Research·Risa TakazakiKazuaki Yoshikawa
Mar 26, 2004·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Hans J BaeldeJan A Bruijn
Aug 3, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuaki Yoshikawa

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