The human colonic thiamine pyrophosphate transporter (hTPPT) is a glycoprotein and N-linked glycosylation is important for its function.

Biochimica Et Biophysica Acta
Svetlana M NabokinaH M Said

Abstract

The recently identified human thiamine pyrophosphate transporter (hTPPT; product of the SLC44A4 gene) is responsible for absorption of the microbiota-generated TPP in the large intestine. The hTPPT is highly expressed in the colon, but not in other regions of the intestinal tract and is localized exclusively at the apical membrane domain of epithelia. The hTPPT protein is predicted to have multiple TM domains with a number of putative N-glycosylation sites, but it is not known if the protein is actually glycosylated, and if so at which site, and their role in the functionality of the transporter. Using several approaches including inhibiting de novo N-glycosylation in human colonic epithelial NCM460 cells with tunicamycin as well as enzymatic de-glycosylation, we show that the hTPPT protein is, indeed, a glycoprotein. Glycosylation of hTPPT was shown, by mean of site-directed mutagenesis, to occur at Asn(69), Asn(155), Asn(197), Asn(393), and Asn(416). However, only N-glycosylation at Asn(69), Asn(155), and Asn(393) appeared to be important for transporter functionality possibly through an effect on protein conformation and/or interaction with its ligand (but not through changes in expression at the cell membrane as determined ...Continue Reading

References

Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·S O'ReganF M Meunier
Aug 30, 2000·Proceedings of the Society for Experimental Biology and Medicine·G Rindi, U Laforenza
Nov 28, 2002·The Journal of Biological Chemistry·Veedamali S SubramanianHamid M Said
Jul 29, 2003·Diabetes·Roya Babaei-JadidiPaul J Thornalley
Nov 15, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hamid M SaidJonathan S Marchant
Jul 16, 2008·Biochemical and Biophysical Research Communications·Veedamali S SubramanianHamid M Said
Jan 20, 2009·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Alan S Hazell, Roger F Butterworth
Jul 14, 2011·The Biochemical Journal·Hamid M Said
Apr 10, 2012·Central Nervous System Agents in Medicinal Chemistry·Vera Michel, Marica Bakovic
May 26, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Svetlana M Nabokina, Hamid M Said
Mar 20, 2013·Molecular Aspects of Medicine·Elisabeth TraiffortMartial Ruat
Aug 31, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hamid M Said
Jan 1, 2014·The Journal of Biological Chemistry·Svetlana M NabokinaHamid M Said

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Citations

May 19, 2016·Expert Review of Neurotherapeutics·Juan Darío Ortigoza-EscobarBelén Pérez-Dueñas
Jun 24, 2017·American Journal of Physiology. Cell Physiology·Veedamali S SubramanianHamid M Said
Nov 20, 2018·Natural Product Reports·Lauren J Rajakovich, Emily P Balskus
Jan 16, 2021·Therapeutic Advances in Psychopharmacology·Michael Ott, Ursula Werneke
Mar 25, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Subrata SabuiHamid M Said

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