The human cytomegalovirus-specific UL1 gene encodes a late-phase glycoprotein incorporated in the virion envelope.

Journal of Virology
Medya ShikhagaieAna Angulo

Abstract

We have investigated the previously uncharacterized human cytomegalovirus (HCMV) UL1 open reading frame (ORF), a member of the rapidly evolving HCMV RL11 family. UL1 is HCMV specific; the absence of UL1 in chimpanzee cytomegalovirus (CCMV) and sequence analysis studies suggest that UL1 may have originated by the duplication of an ancestor gene from the RL11-TRL cluster (TRL11, TRL12, and TRL13). Sequence similarity searches against human immunoglobulin (Ig)-containing proteins revealed that HCMV pUL1 shows significant similarity to the cellular carcinoembryonic antigen-related (CEA) protein family N-terminal Ig domain, which is responsible for CEA ligand recognition. Northern blot analysis revealed that UL1 is transcribed during the late phase of the viral replication cycle in both fibroblast-adapted and endotheliotropic strains of HCMV. We characterized the protein encoded by hemagglutinin (HA)-tagged UL1 in the AD169-derived HB5 background. UL1 is expressed as a 224-amino-acid type I transmembrane glycoprotein which becomes detectable at 48 h postinfection. In infected human fibroblasts, pUL1 colocalized at the cytoplasmic site of virion assembly and secondary envelopment together with TGN-46, a marker for the trans-Golgi net...Continue Reading

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Citations

Jun 7, 2014·Frontiers in Microbiology·Ellen Van Damme, Marnix Van Loock
Apr 7, 2017·European Journal of Immunology·Domènec FarréAna Angulo
Jun 4, 2019·Frontiers in Microbiology·Mathilde FoglieriniLaurent Perez
Aug 9, 2020·Frontiers in Microbiology·Yu-Qing Wang, Xiang-Yu Zhao
Oct 25, 2020·Pathogens·Pearley ChintaJuliet V Spencer

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