PMID: 8972724Nov 8, 1996Paper

The human diploid fibroblast senescence pathway is independent of interleukin-1 alpha mRNA levels and tyrosine phosphorylation of FGFR-1 substrates

Biochimica Et Biophysica Acta
S GarfinkelThomas Maciag

Abstract

In vitro cellular senescence of human umbilical vein endothelial cells (HUVEC) may involve the intracellular activity of the signal peptide-less cytokine interleukin (IL)-1 alpha. To determine whether senescence of other human diploid cells involves the function of IL-1 alpha, we examined the steady-state expression of IL-1 alpha mRNA in IMR-90 fibroblasts. The IL-1 alpha transcript was not elevated in senescent IMR-90 cells. With the exception of the plasminogen activator inhibitor (PAI)-1 transcript, other IL-1 alpha-response gene mRNAs were not induced in senescent IMR-90, although the mRNA for each gene was induced by exogenous IL-1 alpha. The mRNA expression of cell cycle-specific genes demonstrated that Fos and ornithine decarboxylase (ODC) were induced in young and senescent cells in response to both serum and fibroblast growth factor (FGF)-1. Histone (H)3 mRNA was induced by serum in young cells, but not in senescent cells, and FGF-1 failed to induce H3 mRNA in either young or senescent cells. Further, while young IMR-90 populations were able to respond to serum as an initiator of DNA synthesis and cell growth, they did not exhibit a response to exogenous FGF-1. FGF receptor (R)-1 substrates were not tyrosine phosphoryl...Continue Reading

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Citations

Apr 8, 2006·Immunity & Ageing : I & a·Massimo MariottiJeanette A M Maier
Apr 5, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Leah C AbrahamDavid L Kaplan
Feb 13, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Kelly SchultzDebbie Beasley
Jun 3, 2004·Journal of Biomedical Materials Research. Part a·Leah C AbrahamDavid L Kaplan
Aug 9, 2006·Biomaterials·Leah C AbrahamDavid L Kaplan

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