The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans

Nature Communications
Sabina Leanti La RosaBjørge Westereng

Abstract

β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-"omic" analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.

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Citations

May 18, 2020·Microorganisms·Serena SchippaFabio Midulla
Aug 26, 2020·Journal of Industrial Microbiology & Biotechnology·Heidi ØstbyAnikó Várnai
Sep 18, 2020·Nucleic Acids Research·Catherine AuslandYanbin Yin
Aug 12, 2020·Critical Reviews in Food Science and Nutrition·Meng YeWei Liu
Sep 29, 2020·Frontiers in Microbiology·Cheng LongKoen Venema
Mar 15, 2020·Proceedings of the National Academy of Sciences of the United States of America·Leszek MichalakBjørge Westereng
Nov 19, 2019·Frontiers in Pharmacology·Si-Zhe WanXuan Zhu
Feb 13, 2021·Biological Trace Element Research·Nan ZhongJunrui Pei
Nov 15, 2020·Nature Communications·Leszek MichalakBjørge Westereng
Nov 14, 2020·Environmental Microbiology·Jonathon A BriggsHarry Brumer
Mar 6, 2021·Biochemical Society Transactions·Shinya Fushinobu, Maher Abou Hachem
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Hao LiXiaoKang Ma
Mar 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jun GengJens Nielsen
Nov 11, 2020·Journal of Bacteriology·Robert W P Glowacki, Eric C Martens
May 1, 2021·Journal of Personalized Medicine·Irina Grigor'evaMarsel Kabilov
May 27, 2021·Environmental Microbiology Reports·Lauren S McKeeJohan Larsbrink
Jun 18, 2021·Frontiers in Immunology·Lynn van OlstHelga E de Vries
Dec 8, 2020·Current Opinion in Structural Biology·Kazune Tamura, Harry Brumer
Aug 3, 2021·Frontiers in Microbiology·Qian QinSuying Ding
Aug 14, 2021·Frontiers in Nutrition·Uttam Kumar JanaBrett Pletschke
Sep 24, 2021·Nature Chemical Biology·Snehal N ChaudhariA Sloan Devlin
Sep 9, 2021·Molecular Nutrition & Food Research·Isabelle E LoganAdrian F Gombart

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Methods Mentioned

BETA
acetylations
RNAseq
Fluorescent microscopy
surface plasmon resonance
isothermal titration calorimetry
PCR
RNA-seq
size exclusion chromatography
chip

Software Mentioned

MULS
Perseus
Biacore T100
SignalP
ZEN Blue
Gene Ortholog Neighborhood viewer
AcGGM
MicroCal Origin
Chromeleon
MaxQuant

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