PMID: 11328591May 1, 2001Paper

The human homologue of fission Yeast cdc27, p66, is a component of active human DNA polymerase delta

Journal of Biochemistry
K ShikataT Tsurimoto

Abstract

An essential eukaryotic DNA polymerase, DNA polymerase delta (pol delta), synthesizes DNA processively in the presence of proliferating cell nuclear antigen (PCNA). Recently, a 66 kDa polypeptide (p66) that displays significant homology within its PCNA binding domain to that of fission yeast cdc27 was identified as a component of mouse and calf thymus pol delta. Our studies show that p66 interacts tightly with other subunits of pol delta during size fractionation of human cell extracts, and co-immunoprecipitates with these subunits along with PCNA-dependent polymerase activity. Active human pol delta could be reconstituted by co-expressing p125, p50, and p66 recombinant baculoviruses, but not by co-expressing p125 and p50 alone. Interaction studies demonstrated that p66 stabilizes the association between p125 and p50. Pull-down assays with PCNA-linked beads demonstrated that p66 increases the overall affinity of pol delta for PCNA. These results indicate that p66 is a functionally important subunit of human pol delta that stabilizes the pol delta complex and increases the affinity of pol delta for PCNA.

Citations

Jan 27, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Toshiki TsurimotoTakemi Enomoto
Jul 8, 2005·BMC Molecular Biology·J Richard G PohlerEmma Warbrick
Mar 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mark HedglinStephen J Benkovic
May 18, 2016·Nucleic Acids Research·Kouji HirotaShunichi Takeda
Apr 9, 2005·Critical Reviews in Biochemistry and Molecular Biology·Parie Garg, Peter M J Burgers
Jan 11, 2017·Nature Communications·Matteo De MarchAlfredo De Biasio
Mar 10, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomoyuki FukuiShou Waga
Nov 13, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takeo NaritaKouji Hirota
Feb 24, 2007·The Journal of Biological Chemistry·Sufang ZhangMarietta Y W T Lee
May 3, 2002·The Journal of Biological Chemistry·Xiaoqing LuAntero G So
Aug 18, 2017·Critical Reviews in Biochemistry and Molecular Biology·Matteo De March, Alfredo De Biasio
Jul 19, 2002·The Journal of Biological Chemistry·Vladimir P BermudezJerard Hurwitz
Mar 3, 2006·The Journal of Biological Chemistry·Hao LiMarietta Y W T Lee
Nov 7, 2020·Journal of Molecular Biology·Yoshihiro MatsumotoAlan E Tomkinson
Jan 13, 2010·Journal of Molecular Biology·Yuji MasudaKenji Kamiya

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