PMID: 8948445Jun 15, 1995Paper

The human MUC2 mucin apoprotein appears to dimerize before O-glycosylation and shares epitopes with the 'insoluble' mucin of rat small intestine

The Biochemical Journal
N AskerG C Hansson

Abstract

Rabbit antiserum against a synthetic peptide corresponding to a tandemly repeated amino acid sequence in the human intestinal mucin apoprotein MUC2 was used in immunoprecipitation to study the biosynthesis of MUC2 in the colon-carcinoma cell line LS 174T. Under non-reducing conditions, two bands were precipitated, the smaller with an apparent size of about 700 kDa on SDS/PAGE. When analysed by two-dimensional electrophoresis after reduction, the larger band migrated to the same position as the smaller band and was interpreted as a putative disulphide-bond-stabilized dimer. Pulse-chase experiments showed only the monomer after 5 min and the appearance of the putative dimer after 30 min. The MUC2 apoprotein was also precipitated by antisera against the HF-deglycosylated peptides of the two highly glycosylated domains of the 'insoluble' mucin complex of rat small intestine [Carlstedt, Herrmann, Karlsson, Sheehan, Fransson and Hansson (1993) J. Biol. Chem. 268, [18771-18781]. Endoprotease Lys-C cleavage of the immunopurified apoprotein gave a large fragment of about 250 kDa that was detected by both the antiserum against the MUC2 tandem repeat and one of the glycopeptide antisera. This supports the view that the 'insoluble' mucin o...Continue Reading

Citations

Jan 3, 2008·The FEBS Journal·Martin E LidellGunnar C Hansson
Feb 21, 2006·Biochimica Et Biophysica Acta·Mahefatiana AndrianifahananaSurinder K Batra
Apr 2, 2014·The International Journal of Biochemistry & Cell Biology·Camille EhreDavid J Thornton
Mar 14, 2013·BioMed Research International·Marina García-MiguelMarcela A Hermoso
Oct 31, 2012·The International Journal of Biochemistry & Cell Biology·Sumaira Z HasnainDavid J Thornton
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Oct 1, 1996·The Journal of Small Animal Practice·M SpeetiE Westermarck
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Feb 13, 2003·The Journal of Biological Chemistry·Martin E LidellGunnar C Hansson
Jul 16, 2008·Inflammatory Bowel Diseases·Michael A McGuckinGraham Radford-Smith
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M A McGuckinB G Ward
Feb 18, 2011·Gut Microbes·Gunnar C Hansson, Malin Ev Johansson
Apr 2, 2009·Biological Chemistry·Georgios Patsos, Anthony Corfield
Apr 9, 2015·Frontiers in Genetics·Louise E TailfordNathalie Juge
Jun 22, 2006·The Biochemical Journal·Martin E Lidell, Gunnar C Hansson
Oct 11, 2002·The Journal of Biological Chemistry·Klaus GodlGunnar C Hansson
Feb 2, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Rozita NaseriMohammad Hosein Farzaei
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