The human papillomavirus 16 European-T350G E6 variant can immortalize but not transform keratinocytes in the absence of E7

Virology
Melissa TogtemaI Zehbe

Abstract

Human papillomavirus type 16 is commonly implicated in HPV-related cancers. However, only a small number of infected individuals progress to this stage. Epidemiological evidence demonstrated that oncogenic risk is population-specific and variations within the viral oncogene, E6, have been suggested to play a role in these findings. Of focus in this study is the European-T350G variant, which is characterized by an L>V amino acid substitution at residue 83 of the prototype E6 protein. To elucidate the functional effects of this polymorphism, we followed keratinocytes transduced with E-T350G E6 for over 60 passages and compared them to keratinocytes transduced, in parallel, with prototype or Asian-American (Q14H/L83V/H78Y) E6. We found that although E-T350G E6 immortalized transduced keratinocytes in the absence of E7, these cells were not fully transformed. We also found that E-T350G down-regulated E-cadherin compared to the other variants, providing a possible link between its population-based oncogenicity and host genetic variations.

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Citations

Apr 13, 2018·Infectious Agents and Cancer·Jean-Damien Combes, Silvia Franceschi
Jan 25, 2019·The Journal of General Virology·Tsz On LaiPaul Ks Chan
Apr 9, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Robert JacksonIngeborg Zehbe
Dec 24, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Luisa GalatiPaola Di Bonito
Oct 31, 2020·International Journal of Molecular Sciences·Guillem DayerIngeborg Zehbe
Sep 14, 2021·Computational and Structural Biotechnology Journal·Binbin XiHongli Du

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