Nov 26, 2018

The human secretome – the proteins secreted from human cells

BioRxiv : the Preprint Server for Biology
Mathias UhlenSophia Hober

Abstract

The proteins secreted by human tissues (the secretome) are important for the basic understanding of human biology, but also for identification of potential targets for future diagnosis and therapy. Here, we present an annotated list of all predicted secreted proteins (n=2,623) with information about cellular origin and spatial distribution in the human body. A high-throughput mammalian cell factory was established to create a resource of recombinant full-length proteins. This resource was used for phenotypic assays involving β-cell dedifferentiation and for development of targeted proteomics assays. A comparison between host cells, including omics analysis, shows that many of the proteins that failed to be generated in CHO cells could be rescued in human HEK 293 cells. In conclusion, the human secretome has been mapped and characterized and a resource has been generated to facilitate further exploration of the human secretome.

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Mentioned in this Paper

Cell Dedifferentiation
HEK293 Cells
Spatial Distribution
Chinese Hamster Ovary Cell
Mammalian Cell
Human Cell Line
Proteomics
Recombinants
Recombinant Proteins
Antibodies, Monoclonal, Humanized

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