The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.

Genes & Development
S I GundersonR R Burgess

Abstract

A DNA-dependent in vitro transcription system for the human U1 small nuclear RNA (snRNA) promoter has been developed. This in vitro transcription system uses extracts of tissue culture cells to drive transcription of an RNA polymerase II-transcribed snRNA gene. A U1 promoter (-393 to +192) template was constructed in which the sequences from +10 to +171 were replaced with a 179-bp sequence from a G-less cassette. This DNA template thus retained all of the known U1 promoter elements, including the U1 3'-end box (positions +175 to +191), which is responsible for snRNA 3'-end formation. HeLa cell nuclear extracts were shown to drive specific transcription of this promoter by RNA polymerase II. This transcription system has many of the properties observed for wild-type snRNA promoters in vivo. Transcription was shown to initiate at +1 (and -2) relative to the U1 promoter and to efficiently (greater than 90%) form a 3' end corresponding to the 3' end found in the primary transcript of U1 in vivo. The transcription signal is responsive to either deletion or replacement of the U1 distal sequence (enhancer-like) and proximal sequence (TATA-like) elements, as well as the 3'-end box. Additionally, the signal was shown by depletion/replet...Continue Reading

Citations

Jun 15, 1992·Biochimica Et Biophysica Acta·J P QuinnA R Farina
Sep 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·J K ChristmanS C Brooks
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A DvirW S Dynan
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H MessierA Fotedar
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M R RobertsF H Ruddle
Sep 25, 1994·Nucleic Acids Research·M OnoJ D Capra
Feb 15, 1993·European Journal of Biochemistry·I M Willis
Jan 1, 1992·Autoimmunity·R G DalzielJ P Quinn
Mar 24, 2012·Transcription·Justyna ZaborowskaShona Murphy
May 11, 2016·BMC Genomics·Susanta K BehuraMolly Duman-Scheel
Jul 1, 1993·Current Biology : CB·J R Woodgett
Jul 15, 1992·Biochemical and Biophysical Research Communications·W W Zhang, M Yaneva
Dec 6, 1991·Science·J E Dahlberg, E Lund

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