The humoral response to heat shock protein 70 in human and murine Schistosomiasis mansoni

Parasite Immunology
D MoserM Q Klinkert

Abstract

We have expressed two cDNA sequences encoding 121 and 230 amino acids of the C-terminus of the Schistosoma mansoni Hsp 70 in Escherichia coli. The products were synthesized as polypeptides fused to the RNA polymerase of bacteriophage MS2, and their reactivities were tested in ELISAs, using sera from human and murine infections. Anti-Hsp70 antibodies were detected in a significant number of individuals suffering from chronic schistosomiasis mansoni, but not in patients with known recent infections. This, together with the finding that antibodies directed at S. mansoni-specific Hsp70 determinants during the course of infection of experimental mice were not detectable until 5-6 weeks post-infection, suggests that the protein may be a useful marker for distinguishing late and early infections. The diagnostic specificity of Hsp70 was evaluated with sera from humans infected with different schistosome species and other parasitic diseases. While some subjects infected with S. haematobium produced antibodies which recognized the S. mansoni Hsp70, no such antibodies were generated in S. japonicum infected individuals. However, cross-reactive antibodies were elicited in donors with other parasitic diseases such as filariasis and malaria....Continue Reading

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Citations

Jan 1, 1991·Springer Seminars in Immunopathology·D Mazier, D Mattei
Jul 15, 1992·Experientia·J Mollenhauer, A Schulmeister
Apr 1, 1992·Molecular and Biochemical Parasitology·D MoserM Q Klinkert
Aug 1, 1992·Parasitology Today·B Maresca, L Carratù
Jan 1, 2004·Trends in Parasitology·Michael J DoenhoffJoanne V Hamilton
May 20, 1999·International Journal for Parasitology·J C Scott, D P McManus
Mar 21, 2009·Molecular & Cellular Proteomics : MCP·Feng LiuZe-Guang Han
Oct 22, 2005·Infection and Immunity·Khaleda Rahman QaziCarmen Fernández
Aug 13, 2013·PLoS Neglected Tropical Diseases·Steffen HahnelThomas Quack
Jan 18, 2011·International Journal for Parasitology·Saskia DewalickJaap J van Hellemond
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Edward José de OliveiraMário Hiroyuki Hirata
Sep 10, 2002·Memórias do Instituto Oswaldo Cruz·Herminia Y KanamuraRaymond T Damian
Nov 1, 1992·Die Naturwissenschaften·R Felleisen, M Q Klinkert
Aug 7, 2021·Vaccines·Ndibonani Kebonang QokoyiAbidemi Paul Kappo

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