The hydantoin transport protein from Microbacterium liquefaciens.

Journal of Bacteriology
Shun'ichi Suzuki, Peter J F Henderson

Abstract

The gene hyuP from Microbacterium liquefaciens AJ 3912 with an added His6 tag was cloned into the expression plasmid pTTQ18 in an Escherichia coli host strain. The transformed E. coli showed transport of radioisotope-labeled 5-substituted hydantoins with apparent K(m) values in the micromolar range. This activity exhibited a pH optimum of 6.6 and was inhibited by dinitrophenol, indicating the requirement of energy for the transport system. 5-Indolyl methyl hydantoin and 5-benzyl hydantoin were the preferred substrates, with selectivity for a hydrophobic substituent in position 5 of hydantoin and for the l isomer over the d isomer. Hydantoins with less hydrophobic substituents, cytosine, thiamine, uracil, allantoin, adenine, and guanine, were not effective ligands. The His-tagged hydantoin transport protein was located in the inner membrane fraction, from which it was solubilized and purified and its identity was authenticated.

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Citations

Nov 29, 2011·Applied Microbiology and Biotechnology·U EngelJ Rudat
Dec 21, 2010·Journal of Synchrotron Radiation·Simone WeyandAlexander D Cameron
Dec 22, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sergio Martínez-RodríguezJuan Ma García-Ruiz
Dec 5, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tatsuro ShimamuraSo Iwata
Jan 28, 2009·Biochemical and Biophysical Research Communications·Mikio TanabeBernadette Byrne
Nov 22, 2011·Biophysical Journal·Joshua L AdelmanMichael Grabe
Jul 18, 2015·PloS One·Hyun Deok Song, Fangqiang Zhu
Dec 14, 2016·The Journal of Physical Chemistry. B·Hyun Deok Song, Fangqiang Zhu
Aug 2, 2018·European Biophysics Journal : EBJ·Aalishaa A AzamMary K Phillips-Jones
Jun 24, 2014·The EMBO Journal·Katie J SimmonsPeter J F Henderson
Dec 17, 2014·Analytical Chemistry·Antonio N CalabreseAlison E Ashcroft
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pikyee Ma, Mary K Phillips-Jones

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