The hydrodynamic motion of Nanodiscs

Chemistry and Physics of Lipids
Tyler CampStephen G Sligar

Abstract

We present a fluorescence-based methodology for monitoring the rotational dynamics of Nanodiscs. Nanodiscs are nano-scale lipid bilayers surrounded by a helical membrane scaffold protein (MSP) that have found considerable use in studying the interactions between membrane proteins and their lipid bilayer environment. Using a long-lifetime Ruthenium label covalently attached to the Nanodiscs, we find that Nanodiscs of increasing diameter, made by varying the number of helical repeats in the MSP, display increasing rotational correlation times. We also model our system using both analytical equations that describe rotating spheroids and numerical calculations performed on atomic models of Nanodiscs. Using these methods, we observe a linear relationship between the experimentally determined rotational correlation times and those calculated from both analytical equations and numerical solutions. This work sets the stage for accurate, label-free quantification of protein-lipid interactions at the membrane surface.

Citations

Nov 10, 2020·Protein Science : a Publication of the Protein Society·Stephen G Sligar, Ilia G Denisov
Aug 5, 2020·Analytical Biochemistry·Mark A McLeanStephen G Sligar
Jun 16, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Thirupathi RavulaAyyalusamy Ramamoorthy
Dec 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Thirupathi Ravula, Ayyalusamy Ramamoorthy

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