PMID: 5257121Jun 1, 1969Paper

The hydrolysis of uridine cyclic phosphonate catalyzed by ribonuclease-A: implications for the mechanism of action of the enzyme

Proceedings of the National Academy of Sciences of the United States of America
M R HarrisJ G Moffatt

Abstract

Uridine 2',3'-cyclic phosphonate (I) is slowly hydrolyzed by ribonuclease-A with k(2) and K(m) values at pH6 that are respectively 1900 and 15 times smaller than the same parameters at the same pH for the related phosphate (II). Since the ratio of rate constants for hydroxide ion catalyzed hydrolysis is about 4, this result is consistent with, but does not prove a mechanism for the enzymic reaction that requires a pseudorotation.

References

May 1, 1968·Biochemistry·D G AndersonF G Walz
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D C WardE Reich
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G C RobertsO Jardetzky
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D A Usher
Dec 5, 1966·Journal of the American Chemical Society·J E Erman, G G Hammes

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Citations

Jan 21, 2017·ACS Medicinal Chemistry Letters·Yuan XiongNathanael S Gray

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