The hydrophobic patch of ubiquitin is required to protect transactivator-promoter complexes from destabilization by the proteasomal ATPases.

Nucleic Acids Research
Chase T Archer, Thomas Kodadek

Abstract

Mono-ubiquitylation of a transactivator is known to promote transcriptional activation of certain transactivator proteins. For the Sacchromyces cerevisiae transactivator, GAL4, attachment of mono-ubiquitin prevents destabilization of the DNA-transactivator complex by the ATPases of the 26S proteasome. This inhibition of destabilization depends on the arrangement of ubiquitin; a chain of ubiquitin tetramers linked through lysine 48 did not display the same protective effect as mono-ubiquitin. This led to an investigation into the properties of ubiquitin that may be responsible for this difference in activity between the different forms. We demonstrate the ubiquitin tetramers linked through lysine 63 do protect from proteasomal-mediated destabilization. In addition, we show that the mutating the isoleucine residue at position 44 interferes with proteasomal interaction in vitro and will abolish the protective activity in vivo. Together, these data implicate the hydrophobic patch of ubiquitin as required to protect transactivators from destabilization by the proteasomal ATPases.

References

Jan 1, 1976·Journal of Bacteriology·O M Kew, H C Douglas
Apr 5, 1987·Journal of Molecular Biology·S Vijay-KumarW J Cook
Feb 18, 1994·Journal of Molecular Biology·W J CookC M Pickart
Mar 1, 1994·Molecular and Cellular Biology·D R ChowdaryH L Ozer
Dec 15, 1995·The Journal of Biological Chemistry·Q DeverauxM Rechsteiner
May 1, 1997·Molecular and Cellular Biology·W V Ding, S A Johnston
Feb 26, 1998·Cell·W BaumeisterE Seemüller
Apr 16, 1998·Biochemistry·R E BealC M Pickart
Jul 9, 1998·Journal of Molecular Biology·P BayerJ Becker
Jan 21, 2001·The Journal of Biological Chemistry·S J Russell, S A Johnston
Jun 16, 2001·The Journal of Biological Chemistry·K E Sloper-MouldL Hicke
Apr 20, 2002·Science·Fernando GonzalezStephen Albert Johnston
Dec 4, 2002·Journal of Molecular Biology·Ranjani VaradanDavid Fushman
Feb 18, 2004·Molecular Cell·Elena Ezhkova, William P Tansey
Apr 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Thomas G GilletteThomas Kodadek
Feb 8, 2006·Nature Structural & Molecular Biology·Sangho LeeJames H Hurley
Mar 7, 2006·Nucleic Acids Research·Rita SulahianThomas Kodadek
Jul 14, 2006·The Journal of Biological Chemistry·Devanjan SikderThomas Kodadek
Dec 15, 2006·Genes & Development·Anwarul FerdousStephen Albert Johnston
Sep 27, 2007·Journal of the American Chemical Society·Bo LiuThomas Kodadek
Jan 25, 2008·Molecular Immunology·Kavita P BhatSusanna F Greer
Mar 8, 2008·The Journal of Biological Chemistry·Chase T ArcherThomas Kodadek
May 24, 2008·Nature·Koraljka HusnjakIvan Dikic
Jun 3, 2008·The Journal of Biological Chemistry·Chase T ArcherThomas Kodadek

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Citations

Feb 15, 2011·The Journal of Biological Chemistry·Amberlyn M WandsAnna K Mapp
Apr 6, 2012·PLoS Biology·Keven AngNorbert Lehming
Mar 3, 2012·Cellular and Molecular Life Sciences : CMLS·Márcio S BaptistaPatrícia Maciel
Feb 18, 2011·The Biochemical Journal·Mei Kee LimNorbert Lehming
Aug 14, 2012·Transcription·Gary Ee, Norbert Lehming
Mar 4, 2021·Physical Chemistry Chemical Physics : PCCP·Christopher PäslackMatthias Heyden

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Methods Mentioned

BETA
immunoprecipitation
PCR
ChIP
cross-linking studies

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