PMID: 8598272Jan 1, 1996Paper

The hydrophobic surface protein layer of enteroaggregative Escherichia coli strains

FEMS Microbiology Letters
S N WaiK Amako

Abstract

The surface of three strains of enteroaggregative Escherichia coli (EAggEC) and three strains of enteropathogenic E. coli (EPEC) were examined using the freeze-substitution technique of electron microscopy and as a result an electron dense surface layer was found only on EAggEC strains but not on EPEC strains. The analysis of the outer membrane proteins by polyacrylamide gel electrophoresis revealed the existence of a unique 38 kDa protein in EAggEC strains. The protein could be easily extracted from the bacterial surface with 5 M LiCl treatment at room temperature. The antiserum raised in mice with 38 kDa protein extracted from the electrophoresed gel was immunoreacted with the surface of the bacteria of EAggEC by immunoelectron microscopy. The hydrophobic surface character of the EAggEC strains was lost after the extraction of the protein layer by LiCl. We thus conclude that the surface protein layer therefore plays an important role in the expression of the aggregative phenotype in EAggEC strains.

References

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Citations

Jun 11, 1998·Emerging Infectious Diseases·J P NataroR L Guerrant
Dec 28, 2007·Infection and Immunity·Naoko ImutaYoshifumi Kawano
May 11, 2010·International Journal of Medical Microbiology : IJMM·Fabiola AvelinoJorge A Girón
Jul 11, 2012·FEMS Immunology and Medical Microbiology·Teresa Estrada-Garcia, Fernando Navarro-Garcia
Sep 18, 2003·FEMS Microbiology Letters·Cristiano G MoreiraWaldir P Elias
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
Apr 30, 1999·FEMS Microbiology Letters·C RichC Forestier
Nov 6, 2002·The Journal of Clinical Investigation·Jalaluddin SheikhJames P Nataro
May 1, 2001·Clinical and Diagnostic Laboratory Immunology·K IidaS Yoshida
Jan 22, 1998·Applied and Environmental Microbiology·P F PérezG L De Antoni
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper

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