PMID: 9525590Apr 3, 1998Paper

The I domain is required for efficient plasma membrane binding of human immunodeficiency virus type 1 Pr55Gag

Journal of Virology
S SandefurPaul Spearman

Abstract

The interaction of the human immunodeficiency virus type 1 (HIV-1) Pr55Gag molecule with the plasma membrane of an infected cell is an essential step of the viral life cycle. Myristic acid and positively charged residues within the N-terminal portion of MA constitute the membrane-binding domain of Pr55Gag. A separate assembly domain, termed the interaction (I) domain, is located nearer the C-terminal end of the molecule. The I domain is required for production of dense retroviral particles, but has not previously been described to influence the efficiency of membrane binding or the subcellular distribution of Gag. This study used a series of Gag-green fluorescent protein fusion constructs to define a region outside of MA which determines efficient plasma membrane interaction. This function was mapped to the nucleocapsid (NC) region of Gag. The minimal region in a series of C-terminally truncated Gag proteins conferring plasma membrane fluorescence was identified as the N-terminal 14 amino acids of NC. This same region was sufficient to create a density shift in released retrovirus-like particles from 1.13 to 1.17 g/ml. The functional assembly domain previously termed the I domain is thus required for the efficient plasma membra...Continue Reading

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Citations

Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PatnaikJ W Wills
Jan 31, 2002·AIDS·H G Göttlinger
Mar 22, 2002·Journal of Virology·Ahmad KhorchidLawrence Kleiman
Jan 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Marilyn D Resh
Dec 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chun TangMichael F Summers
Mar 18, 2004·Reviews in Medical Virology·Catherine S Adamson, Ian M Jones
Oct 28, 2004·Journal of Virology·Delphine MuriauxAlan Rein
Mar 15, 2006·The Journal of Cell Biology·Amy M BoothStephen J Gould
Nov 6, 2007·Journal of Virology·Scott P KenneyLeslie J Parent
Mar 29, 2003·Journal of Virology·Michael J BrignatiRichard J Courtney
Nov 18, 2003·Traffic·Sascha NydeggerMarkus Thali
Apr 29, 2005·Journal of Virology·Amanda K DaltonVolker M Vogt
Jul 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jamil S SaadMichael F Summers
Jan 5, 2002·Applied Immunohistochemistry & Molecular Morphology : AIMM·J J WangL Ratner
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhibin WuWuyuan Lu

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