The Ice Nucleation Gene from Pseudomonas syringae as a Sensitive Gene Reporter for Promoter Analysis in Zymomonas mobilis.

Applied and Environmental Microbiology
C DrainasN J Panopoulos

Abstract

The expression of the ice nucleation gene inaZ from Pseudomonas syringae in Zymomonas mobilis strains under the control of three different promoters was investigated to establish the utility of the gene as a reporter and examine the possible use of the organism as a source of ice nuclei for biotechnological applications. A promoterless version of the inaZ gene was placed under the control of three different promoters: P(infpdc) (pyruvate decarboxylase), a homologous strong promoter from Z. mobilis; P(infbla) ((beta)-lactamase) of plasmid pBR325; and P(infhrpR), the promoter of hrpR, a regulatory gene from P. syringae pv. phaseolicola. The apparent strengths of all three promoters, measured by quantifying the ice nucleation activity at -9 deg C, were lower in Z. mobilis than in Escherichia coli. The levels of ice nucleation activity expressed under the P(infpdc) promoter were significantly higher than those obtained with the two heterologous promoters in Z. mobilis. Plasmid pCG4521 (RK2 replicon) gave much lower levels of ice nucleation activity when propagated in strain uvs-51, a plasmid instability mutant of Z. mobilis, compared with the wild-type strain. The ice nucleation activity in Z. mobilis cultures showed unusual partit...Continue Reading

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Citations

Jan 21, 2019·Applied Microbiology and Biotechnology·Kun ZhangHailei Wang
Oct 21, 2016·Microbial Biotechnology·Shihui YangMin Zhang
Feb 15, 2020·Biomolecules·Aneta BiałkowskaAleksandra Twarda-Clapa
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daehwan Kim, Seockmo Ku
Apr 9, 2018·Metabolic Engineering·Xia WangShihui Yang

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