PMID: 8978086Dec 15, 1996Paper

The identification a novel gene required for lipopolysaccharide biosynthesis by Haemophilus influenzae RM7004, using transposon Tn916 mutagenesis

FEMS Microbiology Letters
N J HighE Richard Moxon

Abstract

Mutagenesis with the transposon Tn916 was used as a strategy to identify genes required for synthesis of the Gal alpha (1-4) beta Gal component of Haemophilus influenzae strain RM7004 lipopolysaccharide. Insertion of Tn916 into an open reading frame (ORF) encoding a protein with 75% homology to the Escherichia coli methionine related protein (Mrp) is described. Mutations in mrp resulted in loss of reactivity with monoclonal antibody (mAb) 4C4, which recognises Gal alpha (1-4) beta Gal, and expression of LPS with a different electrophoretic profile to that of wild-type RM7004. An unexpected feature of this mutation was that it appeared to influence the number of copies of 5'-CAAT-3' present in lic2A, a gene which is also required for biosynthesis and phase variable expression of the Gal alpha (1-4) beta Gal LPS epitope.

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Citations

Aug 16, 2002·Molecular Microbiology·Laurel L Lenz, Daniel A Portnoy
Sep 13, 2006·Infection and Immunity·Alice L ErwinArnold L Smith
Nov 5, 1999·Microbial Pathogenesis·T E FullerM H Mulks
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A van BelkumH Verbrugh

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