The identification and characterization of oligodendrocyte thromboxane A2 receptors.

The Journal of Biological Chemistry
S C BlackmanG C Le Breton

Abstract

The presence of functional thromboxane A2 receptors in neonatal rat oligodendrocytes and human oligodendroglioma cells was investigated using immunocytochemistry, ligand affinity chromatography, radioligand binding analysis, immunoblot analysis, and calcium mobilization studies. Immunocytochemical studies revealed the presence of receptor protein on both oligodendrocytes and human oligodendroglioma cells. Ligand affinity chromatography allowed for the purification of a protein with an electrophoretic mobility (55 kDa) indistinguishable from human platelet thromboxane A2 receptors. This affinity purified protein was immunoreactive against a polyclonal anti-thromboxane A2 receptor antibody. Intact human oligodendroglioma cells specifically bound [3H]SQ29,548 with a KD of 4 nM and were found to have approximately 3500 binding sites per cell. Human oligodendroglioma cells also demonstrated calcium mobilization in response to receptor activation with U46619. These results demonstrate the presence of a functional thromboxane A2 receptor in oligodendrocytes and are consistent with previous observations indicating a high density of thromboxane A2 receptors in myelinated brain and spinal cord fiber tracts.

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Citations

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May 11, 2002·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·N A SharifS X Xu
Oct 28, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Liang-Wu FuJohn C Longhurst
Apr 1, 2008·Pharmacology & Therapeutics·Norimichi Nakahata
Aug 1, 2006·European Journal of Pharmacology·Shigeyoshi HonmaNorimichi Nakahata
Jul 5, 2005·Neuroscience Letters·Michael J WackerJames A Orr
Apr 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A GieseM Westphal
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