The identification and characterization of expression of Pftaire-1, a novel Cdk family member, suggest its function in the mouse testis and nervous system

Molecular Reproduction and Development
V BessetD J Wolgemuth

Abstract

We have isolated a murine cDNA encoding for a novel putative Cdk-related protein kinase, which has been named Pftaire-1, by screening a testis cDNA library for new serine/threonine kinases. Pftaire-1 showed 50% and 49% amino acid identity with Cdk5 and Pctaire-3, respectively, and contains the eleven subdomains characteristic of the protein kinases. By northern blot analysis we detected two transcripts of approximately 5.5 and 4.9 kb in size. These transcripts were expressed at low level in all murine tissues tested, except in the brain, testis and embryo, where high expression was detected. Cellular localization of the mRNAs by in situ hybridization analysis shows that Pftaire-1 is expressed in late pachytene spermatocytes in the testis and in post mitotic neuronal cells both in the brain and the embryo, suggesting a role of Pftaire-1 both in the process of meiosis as well as neuron differentiation and/or function.

References

Nov 1, 1992·Molecular Reproduction and Development·D L Chapman, D J Wolgemuth
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B A BunnellV J Kidd
Nov 10, 1995·The Journal of Biological Chemistry·D TangJ H Wang
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
Oct 1, 1994·Developmental Biology·D L Chapman, D J Wolgemuth
Dec 24, 1993·Science·S van den Heuvel, E Harlow
Dec 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K Rhee, D J Wolgemuth
Dec 1, 1995·Developmental Biology·M SchäferU Schäfer
Jun 14, 1996·The Journal of Biological Chemistry·D SunR K Liem
Jan 1, 1995·Reproduction, Fertility, and Development·D J WolgemuthS E Ravnik
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·T OhshimaA B Kulkarni
Feb 1, 1997·Current Opinion in Genetics & Development·P J Follette, P H O'Farrell
Feb 1, 1997·Current Opinion in Genetics & Development·A W Page, T L Orr-Weaver
Feb 28, 1997·The Journal of Biological Chemistry·K Y LeeJ H Wang
May 20, 1997·Molecular & General Genetics : MGG·F SladeczekF Le Bouffant
Jul 1, 1997·Journal of Neurochemistry·M A LazzaroJ P Julien
Mar 1, 1957·The Journal of Experimental Zoology·B MINTZ, E S RUSSELL
Mar 27, 2007·Cellular Microbiology·Daniel E Voth, Robert A Heinzen

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Citations

Aug 14, 1999·European Journal of Biochemistry·L DétivaudL Meijer
Dec 21, 2011·Molecular and Cellular Biology·Petra MikolcevicStephan Geley
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fang ShuJun Wu
Aug 4, 2015·Journal of Molecular Neuroscience : MN·Shaochen FanTao Tao
Feb 1, 2002·The Journal of Biological Chemistry·Ted O'NeillGary A Rathbun
Jul 13, 2006·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Jose RussoIrma H Russo
Jun 16, 2009·FEBS Letters·Mei JiangJiangye Chen
Jul 17, 2014·The Journal of Biological Chemistry·Jimcy PlatholiHugh C Hemmings
Dec 25, 2010·Molecular and Cellular Biochemistry·Wilson K C LeungNathalie Wong
Oct 31, 2014·Journal of Molecular Neuroscience : MN·Hai Jie YangZhi Wei Feng
Mar 7, 2006·Acta Biochimica Et Biophysica Sinica·Yan-Kun GaoJiang-Ye Chen
Aug 12, 2010·Fly·Dongmei LiuRussell L Finley
Jan 5, 2002·Insect Biochemistry and Molecular Biology·C S Thummel
Jun 5, 2003·Mechanisms of Development·Anne RascleDavid S Hogness

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