The identification and isolation of CTCs: A biological Rubik's cube

Critical Reviews in Oncology/hematology
Cristina MansillaNatalia Ramírez

Abstract

Liquid biopsy represents an alternative to conventional biopsies for the evaluation of tumors mainly due to its easy sampling. One of the main applications is the enumeration of Circulating Tumor Cells (CTCs) to evaluate tumor progression or response to treatment. The analysis of the functional characteristics of CTCs could give us much more information about their role in order to establish a more personalized treatment for the patients. The major issue that has to be solved is the isolation of the CTC population. Multiple protocols have been developed, however none of them has demonstrated to be the definitive one. In fact, a combination of these techniques has often been performed in order to obtain a purer and viable population of CTCs. In this review we have summarized for the first time the different combinatorial approaches used in the last years to optimize the isolation of CTCs and their limitations.

Citations

Sep 6, 2018·Biology of Reproduction·Aymara Mas, Carlos Simón
Oct 31, 2018·American Journal of Clinical Dermatology·Russell J DiefenbachHelen Rizos
Dec 28, 2018·NPJ Precision Oncology·Erin R BonnerJavad Nazarian
Dec 15, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mariano Di TrapaniGianni Medoro
May 30, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·P ZhangT Feng
Aug 28, 2019·Therapeutic Advances in Medical Oncology·Ugo De GiorgiJames M Reuben
Apr 17, 2021·Critical Reviews in Oncology/hematology·Tito Palmela LeitãoLuís Costa

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