PMID: 140917Jun 1, 1977

The identification in adult bone marrow of pluripotent and restricted stem cells of the myeloid and lymphoid systems

The Journal of Experimental Medicine
S AbramsonR A Phillips

Abstract

The precise relationship between the stem cells for the lymphoid system and those for the blood-forming system is unclear. While it is generally assumed that the hemopoietic stem cell, the spleen colony-forming unit (CFU-S), is also the stem cell for the lymphoid system, there is little evidence for this hypothesis. To investigate the stem cells in these two systems, we irradiated bone marrow cells to induce unique chromosome aberrations in the stem cell population and injected them at limiting dilution into stem cell-deficient recipients. Several months (between 3 and 11) were allowed for the injected cells to repopulate the hemopoietic system. At that time, the bone marrow, spleen, and thymus were examined for a high frequency of cells having the same unique chromosome aberration. The presence of such markers shows that the marker was induced in a cell with extensive proliferative capacity, i.e., a stem cell. In addition, the splenic lymphocytes were stimulated with phytohemagglutinin (PHA) or lipopolysaccharide (LPS) to search for unique chromosomes in dividing T and B cells, respectively. Finally, bone marrow cells were injected into secondary irradiated recipients to determine if the marker occurred in CFU-S and to determi...Continue Reading

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Related Concepts

Chromosome Markers
T-Lymphocyte
Colony-Forming Units, Hematopoietic
Pluripotent Stem Cells
Spleen Colony-forming Unit
Spleen
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Neoplasm of Uncertain or Unknown Behavior of Thymus
Bone Marrow
Lymphocyte Culture Test, Mixed

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