The identification of a novel Sulfolobus islandicus CAMP-like peptide points to archaeal microorganisms as cell factories for the production of antimicrobial molecules

Microbial Cell Factories
Eugenio NotomistaPatrizia Contursi

Abstract

Pathogenic bacteria easily develop resistance to c onventional antibiotics so that even relatively new molecules are quickly losing efficacy. This strongly encourages the quest of new antimicrobials especially for the treatment of chronic infections. Cationic antimicrobial peptides (CAMPs) are small positively charged peptides with an amphipathic structure, active against Gram-positive and Gram-negative bacteria, fungi, as well as protozoa. A novel (CAMP)-like peptide (VLL-28) was identified in the primary structure of a transcription factor, Stf76, encoded by pSSVx, a hybrid plasmid-virus from the archaeon Sulfolobus islandicus. VLL-28 displays chemical, physical and functional properties typical of CAMPs. Indeed, it has a broad-spectrum antibacterial activity and acquires a defined structure in the presence of membrane mimetics. Furthermore, it exhibits selective leakage and fusogenic capability on vesicles with a lipid composition similar to that of bacterial membranes. VLL-28 localizes not only on the cell membrane but also in the cytoplasm of Escherichia coli and retains the ability to bind nucleic acids. These findings suggest that this CAMP-like peptide could exert its antimicrobial activity both on membrane and intra ce...Continue Reading

References

Nov 7, 1998·Biochimica Et Biophysica Acta·K Matsuzaki
Dec 11, 1999·Biochimica Et Biophysica Acta·R M Epand, H J Vogel
Jul 29, 2000·Biochemistry·H W Huang
Aug 12, 2000·Journal of Bacteriology·L B Price, R F Shand
Jan 25, 2002·Nature·Michael Zasloff
Apr 9, 2002·Journal of Industrial Microbiology & Biotechnology·E M O'Connor, R F Shand
Mar 11, 2004·European Journal of Biochemistry·Emma AnderssonArtur Schmidtchen
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Jan 12, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Mohamed Zaiou
Apr 27, 2007·Chembiochem : a European Journal of Chemical Biology·Stefania GaldieroMassimiliano Galdiero
Nov 28, 2007·Expert Review of Anti-infective Therapy·John D F Hale, Robert E W Hancock
Dec 2, 2008·Biochimica Et Biophysica Acta·Ju Hyun ChoSun Chang Kim
Apr 28, 2010·PLoS Pathogens·Praveen PapareddyArtur Schmidtchen
Aug 20, 2010·Environmental Technology·Barbara NicolausEbru Toksoy Oner
Mar 16, 2011·Antimicrobial Agents and Chemotherapy·Gopinath KasettyArtur Schmidtchen
Jun 15, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Annarita FalangaStefania Galdiero
Jul 5, 2011·Journal of Bacteriology·Albert F EllenArnold J M Driessen
Sep 7, 2011·Journal of Molecular Microbiology and Biotechnology·Sylvia A Baltzer, Melissa H Brown
Dec 17, 2011·Nature Reviews. Drug Discovery·Christopher D FjellGisbert Schneider
Jan 31, 2012·Biochimica Et Biophysica Acta·David V Tulumello, Charles M Deber
Jan 4, 2013·Extremophiles : Life Under Extreme Conditions·Baolei JiaShihong Zhang
Aug 1, 2013·International Journal of Nanomedicine·Giovanni SmaldoneEmilia Pedone
Aug 13, 2013·International Journal of Peptides·Muthuirulan PushpanathanJeyaprakash Rajendhran
Sep 3, 2013·Journal of Molecular Recognition : JMR·Stefania CorrealeEmilia Pedone
Nov 30, 2013·Pharmaceuticals·Ali Adem Bahar, Dacheng Ren
Mar 12, 2015·Current Medicinal Chemistry·Stefania GaldieroMassimiliano Galdiero
Mar 15, 2015·Biochimica Et Biophysica Acta·Thitiporn AnunthawanSompong Klaynongsruang

❮ Previous
Next ❯

Citations

May 24, 2016·International Journal of Molecular Sciences·Annarita FalangaStefania Galdiero
Jun 27, 2018·FEMS Microbiology Reviews·Christopher T StraubRobert M Kelly
Jun 7, 2019·Microbiology Resource Announcements·Martina AulittoPatrizia Contursi
Mar 27, 2020·Current Topics in Medicinal Chemistry·Bruna de Oliveira Costa, Octávio Luiz Franco
Feb 1, 2019·World Journal of Microbiology & Biotechnology·Martina AulittoPatrizia Contursi
Nov 5, 2020·MBio·Giuseppe Buda De CesareMichael C Lorenz
Aug 16, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eskandar MoghimipourSomayeh Handali

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
NMR
electrophoretic mobility assay

Software Mentioned

CDPRO
Spectra Manager

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.