The identification of a VDAC-like protein involved in the interaction of Babesia bigemina sexual stages with Rhipicephalus microplus midgut cells

Veterinary Parasitology
Elba Rodríguez-HernándezMinerva Camacho-Nuez

Abstract

We investigated the interaction of Rhipicephalus microplus midgut cells with Babesia bigemina sexual stages using a proteomic approach. A polypeptide from the R. microplus midgut that binds to proteins from B. bigemina sexual stages was identified and sequenced. Combining 2D overlay and tandem mass spectrometry (MS/MS) techniques, we determined that this polypeptide corresponds to a mitochondrial voltage-dependent anion-selective channel (VDAC). The vdac gene encoding the sequenced polypeptide was identified and sequenced. This is the first report of a VDAC-like protein in R. microplus, and a possible role for this protein in the B. bigemina infection process is suggested.

References

May 6, 2003·Biochemical and Biophysical Research Communications·Patricia BoyaGuido Kroemer
Dec 4, 2004·Biochimica Et Biophysica Acta·Patricia BoyaGuido Kroemer
May 16, 2007·Molecular Microbiology·John C Carmen, Anthony P Sinai
Jan 6, 2009·Annals of the New York Academy of Sciences·Juan MosquedaAntonio Alvarez
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel

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Citations

Jul 30, 2014·Parasitology·Monica Florin-ChristensenLeonhard Schnittger
Jul 5, 2015·Veterinary Parasitology·Elba Rodríguez-HernándezMinerva Camacho-Nuez
Aug 2, 2018·Expert Review of Proteomics·Sara Artigas-JerónimoMargarita Villar
Oct 17, 2017·Frontiers in Cellular and Infection Microbiology·Sandra AntunesAna Domingos
Jan 19, 2017·Expert Review of Proteomics·Margarita VillarJosé de la Fuente
Jun 9, 2020·Frontiers in Physiology·Weiqing ZhengXuenan Xuan

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