The identification of four protein kinase C isoforms in human glioblastoma cell lines: PKC alpha, gamma, epsilon, and zeta

Journal of Neurosurgery
H XiaoT Mapstone

Abstract

Levels of protein kinase C (PKC) isoforms in eight human glioblastoma cell lines and two normal human glial cell cultures were determined. Earlier studies identified PKC-alpha and PKC-gamma in these cell lines but PKC-beta was not present. In this study, PKC-epsilon and PKC-zeta are demonstrated immunologically in these cell lines and also in two normal human glial cell cultures. Protein kinase C-delta was not present. When levels of the four isoforms in the tumor cells were compared to levels in the normal cells, no increase was observed in PKC-alpha or PKC-gamma, but PKC-epsilon was elevated three to 30 times in six of the eight tumors, and PKC-zeta was elevated approximately two times in all of the tumors. Incubation of cell line A172 with phorbol ester for 6 hours resulted in a 48-fold maximum increase in the nuclear PKC-epsilon and a sevenfold increase in the plasma membrane fraction with no change in the cytoplasmic fraction. A similar incubation for 4 hours produced a 0.5- to onefold increase of PKC-zeta in cytoplasmic, nuclear, and plasma membrane fractions. Other researchers have shown that overexpression of PKC-epsilon in fibroblasts results in tumorigenesis, and that blocking PKC-zeta function inhibits deoxyribonucle...Continue Reading

References

Jan 1, 1992·Glia·A Misra-PressD A Goldthwait
Dec 15, 1992·Cancer·H H WangM E Kadin
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K OgitaY Nishizuka
Apr 1, 1992·The Journal of Cell Biology·W C WetselY A Hannun
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H KoideY Nishizuka
Sep 15, 1991·European Journal of Biochemistry·A R Olivier, P J Parker
Jan 1, 1990·Neuroscience Letters·S ShimosawaH Hidaka
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y OnoY Nishizuka
Oct 6, 1989·JAMA : the Journal of the American Medical Association·Y Nishizuka
Aug 1, 1993·Molecular and Cellular Biology·M T Diaz-MecoJ Moscat

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Citations

Nov 26, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·K K ChoM L Rosenblum
Jun 1, 1997·Antisense & Nucleic Acid Drug Development·R I Glazer
Jul 17, 2014·International Immunopharmacology·Lisheng GeMartin R Jadus
Aug 17, 2010·Neuroimaging Clinics of North America·Eudocia C Quant, Patrick Y Wen
Nov 18, 2017·International Journal of Molecular Sciences·Shailaja Mahajan-ThakurBernhard H Rauch
Feb 15, 2018·BMC Cancer·Ewa TotonMaria Rybczynska
Jun 7, 2005·Expert Opinion on Therapeutic Targets·Patrick M Martin, Isa M Hussaini
Nov 14, 2018·Journal of Cellular Physiology·Lei TianQingzhang Li
Sep 24, 2002·The Journal of Biological Chemistry·Bakhrom K BerdievDale J Benos
Apr 10, 2003·Journal of Enzyme Inhibition and Medicinal Chemistry·Nidia AlvarezPatrice Le Pape
Aug 11, 2000·Applied Immunohistochemistry & Molecular Morphology : AIMM·R KorenS R Sampson
Mar 31, 2005·The Journal of Pharmacology and Experimental Therapeutics·Brian E ReeseJong Yun
Nov 1, 1995·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·G H BaltuchV W Yong
May 30, 2008·International Journal of Cancer. Journal International Du Cancer·Angus J CameronPeter J Parker

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Methods Mentioned

BETA
PMA
protein gel blot
protein assay
electrophoresis

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