The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization

Anaerobe
Yoshiaki MasakiyoT Takehara

Abstract

Prevotella intermedia and Prevotella nigrescens, which are often isolated from periodontal sites, were once considered two different genotypes of P. intermedia. Although the genomic sequence of P. intermedia was determined recently, little is known about the genetic differences between P. intermedia and P. nigrescens. The subtractive hybridization technique is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains or species. We used subtractive hybridization to identify the DNA regions specific to P. intermedia ATCC 25611 and P. nigrescens ATCC 25261. Using this method, four P. intermedia ATCC 25611-specific and three P. nigrescens ATCC 25261-specific regions were determined. From the species-specific regions, insertion sequence (IS) elements were isolated for P. intermedia. IS elements play an important role in the pathogenicity of bacteria. For the P. intermedia-specific regions, the genes adenine-specific DNA-methyltransferase and 8-amino-7-oxononanoate synthase were isolated. The P. nigrescens-specific region contained a Flavobacterium psychrophilum SprA homologue, a cell-surface protein involved in gliding motility, Prevotella melaninogenica ATCC 25845 glutathione p...Continue Reading

References

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Apr 1, 1996·Oral Microbiology and Immunology·J MättöS Asikainen
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Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·N S AkopyantsD E Berg
Jul 24, 2007·Journal of Bacteriology·Shawn S NelsonMark J McBride

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