The identification of immune genes in the milk transcriptome of the Tasmanian devil (Sarcophilus harrisii)

PeerJ
Rehana V HewavisentiKatherine Belov

Abstract

Tasmanian devil (Sarcophilus harrisii) pouch young, like other marsupials, are born underdeveloped and immunologically naïve, and are unable to mount an adaptive immune response. The mother's milk provides nutrients for growth and development as well as providing passive immunity. To better understand immune response in this endangered species, we set out to characterise the genes involved in passive immunity by sequencing and annotating the transcriptome of a devil milk sample collected during mid-lactation. At mid-lactation we expect the young to have heightened immune responses, as they have emerged from the pouch, encountering new pathogens. A total of 233,660 transcripts were identified, including approximately 17,827 unique protein-coding genes and 846 immune genes. The most highly expressed transcripts were dominated by milk protein genes such as those encoding early lactation protein, late lactation proteins, α-lactalbumin, α-casein and β-casein. There were numerous highly expressed immune genes including lysozyme, whey acidic protein, ferritin and major histocompatibility complex I and II. Genes encoding immunoglobulins, antimicrobial peptides, chemokines and immune cell receptors were also identified. The array of imm...Continue Reading

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Citations

Oct 12, 2016·Scientific Reports·E PeelK Belov
Nov 16, 2019·BMC Genomics·Íria Gabriela Dias Dos SantosErika Cristina Jorge
Jun 20, 2020·Integrative Organismal Biology·B D Fehrenkamp, R D Miller
Mar 23, 2017·Frontiers in Microbiology·Yuanyuan Cheng, Katherine Belov

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Datasets Mentioned

BETA
PRJNA274196
SUB812082
GEDN00000000
AAQ15117
AAL85634
AAB33234.1
AAA93179.1
EX196900.1

Methods Mentioned

BETA
RNAseq

Software Mentioned

FGENESH
TMHMM
IDMM
RSEM
MEGA6
BLASTp
ENSEMBL
SQLite
Trinity
BLAST

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