The identification of myogenic cells in skeletal muscle, with emphasis on the use of tritiated thymidine autoradiography and desmin antibodies

Journal of Anatomy
M J Lawson-Smith, J K McGeachie

Abstract

The identification of myogenic precursor cells (mpc) is a key factor in determining the early events in the myogenesis and regeneration of skeletal muscle. Although satellite cells have long been established as the providers of myoblastic cells, very little is really known (apart from their anatomical location in relation to muscle fibres and their ability to migrate) about the precise role of satellite cells in myogenesis. Numerous techniques for labelling mpc have been devised, but none of these has proven to be completely reliable in firmly establishing the origin of myogenic cells. The use of tritiated thymidine to label DNA in proliferating mpc (which are not specifically distinguishable at the time) and the subsequent location of their labelled progeny in myotube nuclei has revealed a great deal of data on the timing of myogenesis, but not about the nature of mpc themselves. DNA synthesis can also be detected by antibodies to the thymidine analogue, bromodeoxyuridine, and also by antibody staining for proliferating nuclear cell antigen. Like tritiated thymidine, these other markers are not specific for muscle but are general markers for DNA synthesis. In situ hybridisation of various muscle-specific genetic markers and th...Continue Reading

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Citations

Mar 1, 2002·Muscle & Nerve·Linda K McLoon, Jonathan D Wirtschafter
Nov 10, 2011·Cell Biochemistry and Biophysics·Bin ShuLi-qun Zhang
Feb 28, 2003·In Vitro Cellular & Developmental Biology. Animal·J MichalK M Byrne
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Mar 7, 2002·American Journal of Physiology. Cell Physiology·N T Mesires, M E Doumit
Jul 11, 2020·International Journal of Molecular Sciences·Anna CiecierskaTomasz Sadkowski

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