The identification of novel potential injury mechanisms and candidate biomarkers in renal allograft rejection by quantitative proteomics.

Molecular & Cellular Proteomics : MCP
T K SigdelM M Sarwal

Abstract

Early transplant dysfunction and failure because of immunological and nonimmunological factors still presents a significant clinical problem for transplant recipients. A critical unmet need is the noninvasive detection and prediction of immune injury such that acute injury can be reversed by proactive immunosuppression titration. In this study, we used iTRAQ -based proteomic discovery and targeted ELISA validation to discover and validate candidate urine protein biomarkers from 262 renal allograft recipients with biopsy-confirmed allograft injury. Urine samples were randomly split into a training set of 108 patients and an independent validation set of 154 patients, which comprised the clinical biopsy-confirmed phenotypes of acute rejection (AR) (n = 74), stable graft (STA) (n = 74), chronic allograft injury (CAI) (n = 58), BK virus nephritis (BKVN) (n = 38), nephrotic syndrome (NS) (n = 8), and healthy, normal control (HC) (n = 10). A total of 389 proteins were measured that displayed differential abundances across urine specimens of the injury types (p < 0.05) with a significant finding that SUMO2 (small ubiquitin-related modifier 2) was identified as a "hub" protein for graft injury irrespective of causation. Sixty-nine urin...Continue Reading

References

May 1, 1985·Kidney International·R K MaryniakM A Weiss
Feb 13, 1999·Kidney International·L C RacusenY Yamaguchi
Nov 25, 2000·Journal of Autoimmunity·D SánchezH Tlaskalová-Hogenová
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N SeddikiJ C Mazié
Apr 30, 2003·Transplantation·Ruchuang DingManikkam Suthanthiran
Jul 11, 2003·The New England Journal of Medicine·Minnie SarwalOscar Salvatierra
Dec 25, 2003·Journal of the American Society of Nephrology : JASN·Stefan SchaubPeter Nickerson
Mar 26, 2004·Human Immunology·Robert A BrayHoward M Gebel
Jun 24, 2004·American Journal of Physiology. Cell Physiology·Adriana C C GirardiPeter S Aronson
Sep 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Lorraine C RacusenKim Solez
Jul 6, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Gunilla EineckePhilip F Halloran
Dec 2, 2005·The New England Journal of Medicine·Thangamani MuthukumarManikkam Suthanthiran
Sep 26, 2006·Fukushima Journal of Medical Science·Kenya WatanabeYukio Sato
Nov 3, 2006·Expert Review of Proteomics·Stefan SchaubPeter Nickerson
Mar 14, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K SolezJ J Weening
Feb 26, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K SolezM Valente
May 6, 2008·Bioinformatics·Ashoka D PolpitiyaRichard D Smith
May 8, 2008·Clinical Transplantation·Tara K SigdelMinnie Sarwal
Nov 1, 2008·Arthritis and Rheumatism·Masataka KawaiMasataka Kuwana
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dany AnglicheauManikkam Suthanthiran
Jun 2, 2009·Nature Reviews. Immunology·Laurence A Turka, Robert I Lechler
Jul 29, 2009·Advances in Clinical Chemistry·Tara K SigdelMinnie M Sarwal
Dec 2, 2009·Transplantation·Luis F QuintanaJosep M Campistol
Sep 25, 2009·Nature·Fatih OzsolakPatrice M Milos
Oct 13, 2009·Clinical Journal of the American Society of Nephrology : CJASN·George J Schwartz, Dana F Work
Feb 13, 2010·Journal of the American Society of Nephrology : JASN·Xuefeng B LingMinnie M Sarwal
Jun 15, 2010·Proteomics. Clinical Applications·Tara K SigdelMinnie M Sarwal
Oct 12, 2010·The New England Journal of Medicine·Brian J Nankivell, Stephen I Alexander
Dec 1, 2010·Kidney International. Supplement·Johan W de Fijter
Feb 11, 2011·Nature·Eric D GreenUNKNOWN National Human Genome Research Institute
Apr 27, 2011·Proteomics. Clinical Applications·Tara K SigdelMinnie M Sarwal
Jul 13, 2011·Molecular and Cellular Biology·Azariyas A Challa, Branko Stefanovic
Jun 27, 2012·Biomarkers in Medicine·Tara K SigdelMinnie M Sarwal
Jul 18, 2012·The Open Rheumatology Journal·Sashidhar Nakerakanti, Maria Trojanowska
Sep 27, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L LiM M Sarwal
Jun 28, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K S KeslarR L Fairchild

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Citations

Oct 14, 2014·Cell Metabolism·Andrew C ShinChristoph Buettner
Dec 30, 2014·Transplantation Reviews·Song Ong, Roslyn B Mannon
Jan 17, 2015·Frontiers in Medicine·Tara K SigdelMinnie M Sarwal
Dec 17, 2014·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Oriol Bestard, Minnie M Sarwal
Jul 28, 2016·Journal of Proteomics·Pamella Araujo MalagrinoAlexandre Costa Pereira
Aug 2, 2016·Transplantation·Dany AnglicheauPierre Marquet
Sep 30, 2016·International Reviews of Immunology·Helena SáManuel Santos Rosa
Sep 30, 2016·Expert Review of Molecular Diagnostics·Michael NasrMinnie Sarwal
Feb 22, 2017·Current Opinion in Nephrology and Hypertension·Julie HoJohn A Wilkins
Aug 21, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T D Blydt-HansenD S Wishart
Jan 26, 2017·Transplantation·Marina Sirota, Minnie M Sarwal
Mar 25, 2016·World Journal of Transplantation·Wilfried GwinnerDavid Marx
Sep 30, 2016·World Journal of Transplantation·Darlene VigilAntonios H Tzamaloukas
Jan 8, 2017·Journal of the American Society of Nephrology : JASN·Madhav C MenonPeter S Heeger
Jul 10, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Ashley Di MeoGeorge M Yousef
Jul 5, 2018·Molecular Diagnosis & Therapy·L E CrowleyS Chand
Feb 6, 2020·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Hassan Argani
Jan 31, 2020·Proteomics. Clinical Applications·Lei SongKunhong Xiao
Oct 10, 2018·Scientific Reports·Branislav KollarLeonardo V Riella
Apr 18, 2019·PeerJ·So Young Ryu, George A Wendt
Feb 7, 2020·International Journal of Molecular Sciences·Piby PaulJae-Wook Oh
Aug 17, 2016·Clinical Proteomics·Ravi C DwivediJohn A Wilkins
Apr 25, 2019·Journal of Clinical Medicine·Drew WatsonMinnie M Sarwal
Oct 7, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Annemarie WeissenbacherBenedikt M Kessler

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