PMID: 7993373Jun 1, 1994Paper

The identification of nuclear and mitochondrial genes by sequencing randomly chosen clones from a marsupial mammary gland cDNA library

Biochemical Genetics
C Collet, R Joseph

Abstract

To increase the number of genes that can be mapped to the genome of the tammar wallaby (Macropus eugenii), we sequenced 100 randomly chosen clones from a mammary gland cDNA library. Provisional identifications were made of seven nuclear genes and one mitochondrial gene encoding two caseins, beta-galactosidase, acetyl-coenzyme A synthetase, lipoprotein lipase, inorganic pyrophosphatase, an ATP-dependent RNA helicase, and cytochrome c oxidase I. Highly conserved genes, such as that encoding acetyl-coenzyme A synthetase, were easily identified even from cross-kingdom matches. Genes which are highly divergent, however, such as those encoding the mature casein peptides, could not be aligned with homologues in the databases. Even in an organ where there is high mRNA species redundancy, the sequence characterization of expressed sequence tags provides a rapid means of gene identification for mapping purposes.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·Nature Genetics·R WaterstonN Halloran
Feb 13, 1992·Nature·M D AdamsJ C Venter
Feb 1, 1991·Journal of Molecular Endocrinology·C ColletK Nicholas
Jan 1, 1990·Reproduction, Fertility, and Development·C ColletK Nicholas
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 15, 1989·Biochemical and Biophysical Research Communications·C ColletK Nicholas
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Aug 1, 1987·The Journal of Dairy Research·J Bonsing, A G Mackinlay
Nov 1, 1993·The Journal of Heredity·G L WoodleeD W Cooper
Jan 1, 1993·Immunogenetics·M A GroenenM J Giphart
Jan 30, 1993·Gene·M A GroenenJ J van der Poel
Apr 7, 1993·Biochimica Et Biophysica Acta·C Collet, R Joseph

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Citations

Apr 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·G Tosser-KloppF Hatey
Dec 1, 1994·Current Opinion in Biotechnology·C Fields
Dec 11, 1999·Nucleic Acids Research·J QuackenbushJ Upton
Mar 1, 1996·Journal of Child Psychology and Psychiatry, and Allied Disciplines·E SimonoffM Rutter
Aug 10, 2000·Psychological Reports·M A Verdugo

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