The identification, purification, and characterization of CooJ. A nickel-binding protein that is co-regulated with the Ni-containing CO dehydrogenase from Rhodospirillum rubrum.

The Journal of Biological Chemistry
R K Watt, P W Ludden

Abstract

CooJ, a nickel-binding protein from the CO dehydrogenase system of Rhodospirillum rubrum, was purified by immobilized metal affinity chromatography. CooJ is a CO-induced protein predicted to contain a nickel binding motif composed of 16 histidine residues in the final 34 amino acids of the 12.5-kDa protein. When cells grown in the presence of CO were fractionated on an immobilized metal affinity chromatography column and analyzed by SDS-polyacrylamide gel electrophoresis, the major protein observed in the effluent migrated at an apparent molecular mass of 19 kDa. The 19-kDa protein was absent in extracts of cells grown in the absence of CO and the mutant strain, UR294, which lacks a functional cooJ gene. N-terminal sequence analysis confirmed that the 19-kDa protein is the product of the cooJ gene. Purified CooJ was shown to bind four nickel atoms per CooJ monomer with a Kd of 4.3 microM. Other divalent metals competed with the following order of affinity and corresponding Ki: Zn2+ (5 microM) > Cd2+ (19 microM) > Co2+ (23 microM) > Cu2+ (122 microM). CooJ chromatographed on a calibrated Superose 12 gel filtration column eluted at 39 kDa, a position consistent with a multimeric native molecular mass for CooJ.

References

Jan 1, 1989·Analytical Biochemistry·T Reinard, H J Jacobsen
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 1, 1984·Journal of Bacteriology·D BonamP W Ludden
Apr 1, 1995·Journal of Bacteriology·R L KerbyG P Roberts
May 19, 1995·Science·E JabriP A Karplus
Feb 16, 1995·Nature·A VolbedaJ C Fontecilla-Camps
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·C FuR J Maier
Jan 5, 1996·The Journal of Biological Chemistry·Y HeG P Roberts

❮ Previous
Next ❯

Citations

Sep 17, 2013·Archives of Biochemistry and Biophysics·Jodi L BoerRobert P Hausinger
Jan 23, 1999·Current Opinion in Structural Biology·U ErmlerR K Thauer
Sep 14, 2012·Biochemistry·Khadine A HigginsMichael J Maroney
Aug 2, 2011·Metallomics : Integrated Biometal Science·Sachi Okamoto, Lindsay D Eltis
Feb 29, 2000·Journal of Bacteriology·M García-DomínguezJ C Reyes
Jul 5, 2005·Journal of Bacteriology·Anelia Atanassova, Deborah B Zamble
Dec 21, 2010·Bulletin of Environmental Contamination and Toxicology·M YusufA Ahmad
Dec 15, 2004·Critical Reviews in Biochemistry and Molecular Biology·Stephen W Ragsdale
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
Jan 11, 2016·Protein Science : a Publication of the Protein Society·Kenric J Hoegler, Michael H Hecht
Oct 21, 2016·Biochimica Et Biophysica Acta·Barbara ZambelliStefano Ciurli
Apr 28, 2017·Metallomics : Integrated Biometal Science·J TimmC Cavazza
Feb 16, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mériem MerrouchSébastien Dementin
Jun 20, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yi-Bo ZengHongzhe Sun
May 6, 2010·Natural Product Reports·Harini KaluarachchiDeborah B Zamble
Oct 10, 2001·The Journal of Biological Chemistry·H K SongR P Hausinger
Apr 27, 2011·Chemistry : a European Journal·Yi-Bo ZengHongzhe Sun
Jul 8, 2017·Metallomics : Integrated Biometal Science·Javier Jiménez-Lamana, Joanna Szpunar
Jun 9, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·M AlfanoC Cavazza
Feb 7, 2001·The Journal of Biological Chemistry·G J Colpas, R P Hausinger
Feb 2, 2019·Frontiers in Microbiology·Masao InoueYoshihiko Sako
Jul 13, 2019·The Journal of Biological Chemistry·Elizabeth C WittenbornCatherine L Drennan
Jul 27, 1999·Journal of Bacteriology·R K Watt, P W Ludden
Aug 29, 2009·Chemical Reviews·Yanjie Li, Deborah B Zamble
Nov 4, 2009·Biochemistry·Jae-Hun JeoungHolger Dobbek
May 5, 1999·Current Opinion in Chemical Biology·M J Maroney

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.