The Iflaviruses Sacbrood virus and Deformed wing virus evoke different transcriptional responses in the honeybee which may facilitate their horizontal or vertical transmission

PeerJ
Eugene V RyabovDavid J Evans

Abstract

Sacbrood virus (SBV) and Deformed wing virus (DWV) are evolutionarily related positive-strand RNA viruses, members of the Iflavirus group. They both infect the honeybee Apis mellifera but have strikingly different levels of virulence when transmitted orally. Honeybee larvae orally infected with SBV usually accumulate high levels of the virus, which halts larval development and causes insect death. In contrast, oral DWV infection at the larval stage usually causes asymptomatic infection with low levels of the virus, although high doses of ingested DWV could lead to DWV replicating to high levels. We investigated effects of DWV and SBV infection on the transcriptome of honeybee larvae and pupae using global RNA-Seq and real-time PCR analysis. This showed that high levels of SBV replication resulted in down-regulation of the genes involved in cuticle and muscle development, together with changes in expression of putative immune-related genes. In particular, honeybee larvae with high levels of SBV replication, with and without high levels of DWV replication, showed concerted up-regulated expression of antimicrobial peptides (AMPs), and down-regulated expression of the prophenoloxidase activating enzyme (PPAE) together with up-regul...Continue Reading

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Citations

Aug 19, 2016·PLoS Pathogens·Laura M BrutscherMichelle L Flenniken
Dec 12, 2018·Environmental Microbiology·Elizabeth A MurrayBryan N Danforth
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Aug 7, 2021·Insects·Alexander J McMenaminMichelle L Flenniken

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Datasets Mentioned

BETA
PRJEB6511
AF092924
GB48820

Methods Mentioned

BETA
RNA-Seq
PCR
reverse transcription PCR

Software Mentioned

AmiGO
samtools idxstats
edgeR
DESeq
Bowtie2

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