PMID: 9531561May 16, 1998Paper

The Ig superfamily cell adhesion molecule, apCAM, mediates growth cone steering by substrate-cytoskeletal coupling

The Journal of Cell Biology
Daniel M SuterPaul Forscher

Abstract

Dynamic cytoskeletal rearrangements are involved in neuronal growth cone motility and guidance. To investigate how cell surface receptors translate guidance cue recognition into these cytoskeletal changes, we developed a novel in vitro assay where beads, coated with antibodies to the immunoglobulin superfamily cell adhesion molecule apCAM or with purified native apCAM, replaced cellular substrates. These beads associated with retrograde F-actin flow, but in contrast to previous studies, were then physically restrained with a microneedle to simulate interactions with noncompliant cellular substrates. After a latency period of approximately 10 min, we observed an abrupt increase in bead-restraining tension accompanied by direct extension of the microtubule-rich central domain toward sites of apCAM bead binding. Most importantly, we found that retrograde F-actin flow was attenuated only after restraining tension had increased and only in the bead interaction axis where preferential microtubule extension occurred. These cytoskeletal and structural changes are very similar to those reported for growth cone interactions with physiological targets. Immunolocalization using an antibody against the cytoplasmic domain of apCAM revealed a...Continue Reading

References

Oct 1, 1991·The Journal of Cell Biology·E M Tanaka, M W Kirschner
Oct 1, 1991·The Journal of Cell Biology·J H SabryD Bentley
Jul 11, 1991·Nature·J A Theriot, T J Mitchison
Dec 1, 1990·The Journal of Cell Biology·F Keller, S Schacher
Dec 1, 1989·The Journal of Cell Biology·T J DennerllS R Heidemann
Jul 13, 1989·Nature·P LamoureuxS R Heidemann
Nov 1, 1988·Neuron·T Mitchison, M Kirschner
Jan 1, 1995·The Journal of Cell Biology·E Tanaka, M W Kirschner
Oct 1, 1994·Current Opinion in Neurobiology·C H LinP Forscher
Feb 1, 1994·Current Opinion in Neurobiology·P Doherty, F S Walsh
Oct 1, 1993·Current Opinion in Neurobiology·U Rutishauser
Feb 25, 1993·Nature·R W DavenportS B Kater
Jun 1, 1993·The Journal of Cell Biology·C H Lin, P Forscher
Feb 9, 1996·Cell·T J Mitchison, L P Cramer
Jan 1, 1996·Annual Review of Neuroscience·C S Goodman
Nov 15, 1996·Science·M Tessier-Lavigne, C S Goodman
Jan 1, 1996·Molecular and Cellular Neurosciences·P Doherty, F S Walsh
Oct 1, 1996·Current Opinion in Cell Biology·F S Walsh, P Doherty
Aug 1, 1996·Molecular and Cellular Neurosciences·P Doherty, F S Walsh
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·T WilliamsonJ Taylor
Jul 1, 1997·Journal of Neuroscience Research·H Kamiguchi, V Lemmon
Feb 7, 1998·Current Opinion in Neurobiology·T Hasson, M S Mooseker

❮ Previous
Next ❯

Citations

Jul 28, 1999·Cell Motility and the Cytoskeleton·M F Krendel, E M Bonder
Jul 12, 2002·Cell Motility and the Cytoskeleton·Paul C Bridgman
Mar 31, 2005·Molecular and Cellular Neurosciences·Véronique MarthiensRené-Marc Mège
Apr 7, 2004·Trends in Cell Biology·Steven R Heidemann, Denis Wirtz
Nov 18, 1998·Current Opinion in Cell Biology·C G Galbraith, M P Sheetz
Nov 18, 1998·Current Opinion in Cell Biology·C L Adams, W J Nelson
May 6, 1998·Current Opinion in Neurobiology·D M Suter, P Forscher
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Massimiliano StagiThomas Biederer
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Dec 10, 1999·Molecular Biology of the Cell·J H HensonG G Borisy
Jul 9, 1999·Molecular Biology of the Cell·M W RochlinP C Bridgman
Jun 17, 2003·Molecular Biology of the Cell·Boris HinzChristine Chaponnier
Jul 16, 2004·Molecular Biology of the Cell·Julien FalkCatherine Faivre-Sarrailh
Dec 2, 2005·Molecular Biology of the Cell·Olivier ThoumineDaniel Choquet
Aug 22, 2008·Molecular Biology of the Cell·Bingbing WuDaniel M Suter
Jan 23, 2009·Molecular Biology of the Cell·Federico Fuentes, Carlos O Arregui
Nov 26, 2010·Cold Spring Harbor Perspectives in Biology·Erik W DentFrank B Gertler
Jan 7, 2005·Anatomical Science International·Joseph FassJames R Bamburg
Jun 5, 2003·BMC Cell Biology·Justin C YarrowTimothy J Mitchison
Sep 17, 2013·PloS One·Cecile O MejeanPaul Forscher
Dec 4, 2009·PLoS Pathogens·Christoph J Burckhardt, Urs F Greber
Dec 11, 2008·Journal of Visualized Experiments : JoVE·Aih Cheun LeeDaniel Suter
Sep 6, 2014·Journal of Biochemistry·Sawako Yamashiro, Naoki Watanabe
Feb 26, 2016·Frontiers in Cell and Developmental Biology·Iryna Leshchyns'ka, Vladimir Sytnyk
Sep 9, 1999·The Journal of Cell Biology·A Mallavarapu, T Mitchison
Jul 20, 2005·The Journal of Cell Biology·Maik J LehmannWalther Mothes
Dec 6, 2003·The Journal of Cell Biology·Kazunari NishimuraHiroyuki Kamiguchi
May 10, 2006·The Journal of Cell Biology·Kyle E Miller, Michael P Sheetz
Jun 4, 2008·The Journal of Cell Biology·Tadayuki ShimadaNaoyuki Inagaki
Oct 31, 2001·The Journal of Cell Biology·D G Jay
Aug 12, 2015·The Journal of Cell Biology·Yusuke KuboNaoyuki Inagaki
Apr 1, 2015·The Journal of Cell Biology·Dominique SiegenthalerJan Pielage

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.