PMID: 11897983Mar 19, 2002Paper

The IL-5 receptor on human bronchus selectively primes for hyperresponsiveness

The Journal of Allergy and Clinical Immunology
Charles A RizzoJohn A Hey

Abstract

The role of IL-5-induced eosinophilia in airway hyperresponsiveness has been questioned. In addition, eosinophil-independent IL-5-induced airway hyperresponsiveness has been demonstrated in animals. In this study, IL-5 was investigated for direct effects on human bronchial responsiveness. Human muscle preparations were isolated from organ donor and surgical tissue. Bronchus, jejunum, and saphenous vein were incubated for 24 hours in vitro with recombinant human (rh) IL-5. Contractility to acetylcholine (bronchus, jejunum) and phenylephrine (saphenous vein) was then investigated. RT-PCR was used to evaluate IL-5 receptor alpha (IL-5R(alpha)) expression in various tissues and to assess bronchus and saphenous vein eosinophils through use of CCR3 expression. rhIL-5 primed bronchus for an exaggerated contraction to acetylcholine. The acetylcholine concentration that produced 50% of the control maximum response was reduced 17- to 20-fold in bronchus treated with 1 and 10 nmol/L rhIL-5. The lower concentration of 0.1 nmol/L rhIL-5 had no effect. The rhIL-5 effect on bronchial contractility was attenuated by antibodies to IL-5 (TRFK-5; 100 nmol/L) and human IL-5R(alpha) (100 nmol/L). rhIL-5 (10 nmol/L) did not enhance contractility of ...Continue Reading

Citations

Jun 11, 2003·American Journal of Respiratory and Critical Care Medicine·A Barry Kay, Andrew Menzies-Gow
Sep 22, 2007·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Dale R Bergren
Apr 14, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F TakiK Shimokata
Dec 6, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C E BrightlingP Bradding
Apr 4, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Abdelilah Soussi Gounni
Apr 22, 2015·American Journal of Respiratory Cell and Molecular Biology·Becky J ProskocilAllison D Fryer
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Apr 14, 2007·American Journal of Respiratory Cell and Molecular Biology·Krishnan ParameswaranP Gerard Cox
Sep 21, 2018·Frontiers in Physiology·Tabata M Dos SantosIolanda F L C Tibério
Apr 4, 2006·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Dagmar SimonHans-Uwe Simon
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Dec 6, 2019·The Journal of Allergy and Clinical Immunology·Martijn L MansonMikael Adner
Jan 18, 2003·The International Journal of Biochemistry & Cell Biology·Yassine Amrani, Reynold A Panettieri
Oct 1, 2003·Respiratory Physiology & Neurobiology·Hakon Hakonarson, Michael M Grunstein
Nov 2, 2020·European Journal of Pharmacology·Miroslava MolitorisovaSona Franova

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