PMID: 2115869Aug 1, 1990Paper

The ilvIH operon of Escherichia coli is positively regulated

Journal of Bacteriology
J V PlatkoJ M Calvo

Abstract

The ilvIH operon of Escherichia coli (located near min 2) encodes acetohydroxyacid synthase III, an isozyme involved in branched-chain amino acid biosynthesis. A strain with lacZ fused to the ilvIH promoter was constructed. Transposon Tn10 was introduced into this strain, and tetracycline-resistant derivatives were screened for those in which ilvIH promoter expression was markedly reduced. In one such derivative, strain CV1008, beta-galactosidase expression was reduced more than 30-fold. The transposon giving rise to this phenotype inserted near min 20 on the E. coli chromosome. Extract from a wild-type strain contains a protein, the IHB protein, that binds to two sites upstream of the ilvIH promoter (E. Ricca, D. A. Aker, and J. M. Calvo, J. Bacteriol. 171:1658-1664, 1989). Extract from strain CV1008 lacks IHB-binding activity. These results indicate that the IHB protein is a positive regulator of ilvIH operon expression. The gene that encodes the IHB protein, ihb, was cloned by complementing the transposon-induced mutation. Definitive evidence that the cloned DNA encodes the IHB protein was provided by determining the sequence of more than 17 amino acids at the N terminus of the IHB protein and comparing it with the nucleotid...Continue Reading

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Citations

Feb 2, 2008·Biological & Pharmaceutical Bulletin·Tsuyoshi KawashimaMasashi Suzuki
Dec 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Byung-Kwan ChoBernhard Ø Palsson
Jun 3, 2010·Biotechnology Journal·Jin Hwan Park, Sang Yup Lee
May 6, 2019·Critical Reviews in Biotechnology·Ying-Yu WangWei-Guo Zhang
May 25, 2011·Journal of Cellular Biochemistry·Wanyan DengJianping Xie
May 17, 2011·FEMS Microbiology Reviews·Ding Jun JinYan Ning Zhou
Nov 14, 2018·Journal of Bacteriology·Grace M KronerPeter L Freddolino
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin Hwan ParkSang Yup Lee
Dec 21, 2010·Journal of Bacteriology·Benjamin R Hart, Robert M Blumenthal
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Jun 4, 2002·The Journal of Biological Chemistry·Arie B BrinkmanJohn van der Oost
Nov 15, 2014·BMC Systems Biology·Christopher L PlaisierNitin S Baliga
Sep 10, 2002·Journal of Bacteriology·Samina JafriJoseph M Calvo
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