PMID: 9420300Jan 7, 1998Paper

The immediate-early gene product encoded by open reading frame 57 of herpesvirus saimiri modulates gene expression at a posttranscriptional level

Journal of Virology
Adrian WhitehouseD Meredith

Abstract

The herpesvirus saimiri (HVS) immediate-early gene product encoded by open reading frame (ORF) 57 shares limited amino acid homology with HSV-1 ICP27 and Epstein-Barr virus BMLF1, both regulatory proteins. The ORF 57 gene has been proposed to be spliced based on the genome sequence, and here we confirm the intron-exon structure of the gene. We also demonstrate that a cDNA construct of the ORF 57 gene product represses the transactivating capability of the ORF 50a gene product (which is produced from a spliced transcript), but activates that of ORF 50b (an unspliced transcript). Further analyses with cotransfection experiments show that ORF 57 can either activate or repress expression from a range of both early and late HVS promoters, depending on the target gene. These results indicate that repression of gene expression mediated by the ORF 57 gene product is dependent on the presence of an intron within the target gene encoding region. Furthermore, Northern blot analysis demonstrates that the levels of mRNA transcribed from genes not containing an intron are not significantly affected in the presence of the ORF 57 gene product. This suggests that it regulates gene expression through a posttranscriptional mechanism.

References

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Citations

Mar 30, 2001·The Journal of Biological Chemistry·D J Goodwin, A Whitehouse
Sep 25, 2004·Journal of Cellular Physiology·Alexander Y Tsygankov
Sep 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·James R Boyne, Adrian Whitehouse
Feb 17, 2009·The Journal of General Virology·Kevin J ColganAdrian Whitehouse
May 13, 2003·Reviews in Medical Virology·Zhi-Ming Zheng
Nov 26, 2008·Veterinary Research Communications·T Leenadevi, R G Dalziel
Nov 24, 1999·The Journal of General Virology·L J BelloJ Barklie Clements
Feb 22, 2005·The Journal of General Virology·Matthew S WaltersAdrian Whitehouse
Oct 21, 2006·Biochemical Society Transactions·P Malik, E C Schirmer
May 10, 2005·Journal of Cellular Biochemistry·Sankar Swaminathan
Dec 25, 2010·Acta Microbiologica Et Immunologica Hungarica·J RajčániS Szathmary
Nov 26, 2008·Veterinary Research Communications·T Leenadevi, R G Dalziel
Jan 31, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J R Boyne, A Whitehouse

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