The immune suppressive microenvironment of human gliomas depends on the accumulation of bone marrow-derived macrophages in the center of the lesion

Journal for Immunotherapy of Cancer
Laura PintonSusanna Mandruzzato

Abstract

Systemic and local immune suppression plays a significant role in glioma progression. Glioma microenvironment contains both brain-resident microglial cells (MG) and bone marrow-derived macrophages (BMDM), but the study of their functional and immune regulatory activity has been hampered until now by the lack of markers allowing a proper identification and isolation to collect pure populations. Myeloid and lymphoid infiltrate were characterized in grade II, III and IV gliomas by multicolor flow cytometry, along with the composition of the cell subsets of circulating myeloid cells. Macrophages were sorted and tested for their immunosuppressive ability. Moreover, following preoperative administration of 5-aminolevulinic acid to patients, distinct areas of tumor lesion were surgically removed and analyzed, based on protoporphyrin IX fluorescence emission. The immune microenvironment of grade II to grade IV gliomas contains a large proportion of myeloid cells and a small proportion of lymphocytes expressing markers of dysfunctional activity. BMDM and resident MG cells were characterized through a combination of markers, thus permitting their geographical identification in the lesions, their sorting and subsequent analysis of the fun...Continue Reading

References

Sep 1, 1996·Acta Neuropathologica·W RoggendorfW Paulus
Oct 16, 2002·Glia·Manuel B GraeberGeorg W Kreutzberg
May 20, 2008·Biochimica Et Biophysica Acta·D R RichardsonD B Lovejoy
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susanna MandruzzatoPaola Zanovello
Dec 30, 2009·European Journal of Immunology·Stefania RecalcatiGaetano Cairo
Dec 1, 2010·Journal of Neuro-oncology·Xinmei ZhuHideho Okada
Nov 16, 2012·Bulletin of Mathematical Biology·Alicia Martínez-GonzálezVíctor M Pérez-García
Apr 25, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Orin BlochAndrew T Parsa
Sep 4, 2014·Cell Biochemistry and Biophysics·Peng DingLiping Xue
Nov 19, 2014·Frontiers in Cellular Neuroscience·Anna Carolina Carvalho da FonsecaFlavia Regina Souza Lima
Jul 29, 2015·Brain, Behavior, and Immunity·Patrícia DominguesMaría Dolores Tabernero
Mar 15, 2016·JCI Insight·Konrad GabrusiewiczAmy B Heimberger
Jan 17, 2017·Nature Immunology·Britta EngelhardtRoy O Weller
May 23, 2017·Neuro-oncology·Anna Sophie BerghoffMatthias Preusser
Jun 14, 2017·The Journal of Experimental Medicine·Amit A PatelSimon Yona
Nov 21, 2017·Nature Reviews. Immunology·Qingyun Li, Ben A Barres

❮ Previous
Next ❯

Citations

Jan 25, 2020·International Journal of Molecular Sciences·Yolanda Pires-AfonsoAlessandro Michelucci
Apr 24, 2020·American Society of Clinical Oncology Educational Book·Leland S HuGlenn J Lesser
Apr 19, 2020·Journal for Immunotherapy of Cancer·Matheus H W CrommentuijnYvette van Kooyk
Jul 10, 2020·Neuro-oncology Advances·Darya S NesterovaJames R Connor
Sep 10, 2020·Journal of Personalized Medicine·Hassan Rahimi KoshkakiAurelia Rughetti
Aug 28, 2020·Expert Review of Anticancer Therapy·Jacob S Young
Apr 23, 2020·International Journal of Molecular Sciences·Stephanie Sanders, Waldemar Debinski
Sep 20, 2020·Journal for Immunotherapy of Cancer·Liang WangHong-Yan Qin
Sep 18, 2020·International Journal of Molecular Sciences·Mario CacceseGiuseppe Lombardi
Jan 8, 2021·Frontiers in Immunology·Yin Qiu TanHong Bo Zhang
Dec 31, 2020·Cells·Alessandra De LeoFilippo Veglia
Mar 7, 2021·Cancers·Hong-My NguyenDipongkor Saha
Mar 18, 2021·OncoTargets and Therapy·Qiyan WangGuishan Jin
May 1, 2021·International Journal of Molecular Sciences·Natalia Di IanniSerena Pellegatta
Jun 22, 2021·Frontiers in Oncology·Hamza AliMyron G Best
Jul 7, 2021·The Journal of Clinical Investigation·Apeng ChenBaoli Hu
Aug 15, 2020·Mayo Clinic Proceedings. Innovations, Quality & Outcomes·Rawan Al-KharbooshAlfredo Quiñones-Hinojosa
Oct 12, 2021·Pathology Oncology Research : POR·Xinzhuang WangXu Hou

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
FACS
flow cytometry
scRNAseq
scRNA-seq

Software Mentioned

RStudio Integrated Development for R
Sigmaplot

Related Concepts

Related Feeds

Cancer Immunotherapy

Cancer immunotherapy is an important field of research that is looking at controlling cancer and tumor growth by activating the individuals own immune system. Recent studies have utilized chimeric antigen receptor t-cell therapy, immune checkpoint inhibitors and neoantigen vaccines. Discover the latest research on cancer immunotherapy here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Ronan KapetanovicDavid A Hume
© 2022 Meta ULC. All rights reserved